Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Añadir filtros








Intervalo de año
1.
Chinese Journal of Biotechnology ; (12): 51-54, 2002.
Artículo en Chino | WPRIM | ID: wpr-231374

RESUMEN

RNAs of the leaves in Avicennia marina, which cultured in 50@1000 and 0@1000 salinity condition respectively, were isolated for mRNA differential display analysis. Screened by OligodT12 GC and eight 10-oligonucleotide arbitrary primers, differential cDNA fragments-csrg1(600 bp), csrg2(550 bp), csrg3(480 bp), only appeared in the leaves of Avicennia marina cultured in 50@1000 salinity condition. After detected by RNA dot hybridization, only csrg1 appeared the difference between the RNAs of the leaves in Avicennia marina cultured in 50@1000 and 0@1000 salinity condition respectively, and csrg1 was confirmed as the salt-tolerant cDNA. csrg1 was cloned and sequenced. After searching in Genbank, there were no similar sequences reported.


Asunto(s)
Secuencia de Bases , Clonación Molecular , ADN Complementario , ADN de Plantas , Perfilación de la Expresión Génica , Métodos , Regulación de la Expresión Génica de las Plantas , Magnoliopsida , Dosis Máxima Tolerada , Hojas de la Planta , Genética , Fisiología , Sales (Química) , Farmacología , Análisis de Secuencia de ADN , Métodos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA