Risk prediction genetic study of non-HLA gene NOD2 three polymorphisms in acute graft versus host disease after allogeneic hematopoietic stem cell transplantation

Background: Graft-versus-host disease [GVHD] has been associated with non- human leukocyte antigen [HLA] gene polymorphisms after allogenic Hematopoietic stem cell transplantation [aHSCT]

Objective: This study aims to investigate the role of three polymorphisms in Nucleotide-binding Oligomerisation Domain [NOD2] gene on the occurrence / severity of acute GVHD among Egyptian population

Methodology: A total of 64 patients and their corresponding donors who underwent aHSCT from HLA-identical matched siblings were genotyped by polymerase chain reaction and restriction fragment length polymorphism [PCR-RFLP]. Results were confirmed by automated laser sequencer

Results: There was highly statistical significant association between NOD2 recipient l007fs allele and higher incidence of aGVHD p= 0.006. There was no significant association between NOD2 donor l007fs allele, G908R allele in donors and recipients, R702W in donors and recipients with incidence and severity of aGVHD

Conclusion: This research an original pilot study to demonstrate that the NOD2 SNP13 [Leu1007fs] is a possible risk factor for aGVHD among Egyptian population

Control and prevention of parasitic diseases among school children and their mothers in rural area

The aim of the present study was to assess knowledge and health habits needs of the school children and their mothers regarding controlling and preventing of the intestinal parasitic diseases, plan and implement an educational health program for school children and their mothers according to their needs, data obtained and reviewing literature and evaluate the effect of the health educational program on the knowledge and health habits practices of the school children and their mothers regarding controlling and preventing of the intestinal parasitic diseases. The study conducted into two areas, the parasitic outpatients clinics of the school health insurance hospital at the waiting areas, and field visits for 50% [50] of infected children's home for assessing their home environment in Benha city. A quasi experimental design was utilized to conduct the study. The study included [100] child with parasitic infection with their mothers. Three tools used for data collection, an interviewing questionnaire, to assess school children's and their mothers' socio-demographic data, children's knowledge, and health habits of children and their mothers, an observational checklist to assess the home environment of the infected children, and the medical record of the children that include the type of intestinal parasitosis according to stool analysis result. The findings showed that after implementation of the health education program there was a statistical significant improvement regarding the knowledge and health habits practices of the school children and their mothers regarding controlling and preventing of the intestinal parasitic diseases. The study recommended that, continuous health education for school children and their families based up on their needs regarding knowledge, healthy habits, and home environmental sanitation about control and prevention of the parasitic diseases

Effects of obesity on self concept, quality of life, and depression among adolescents

Pediatric overweight is a national epidemic with significant long term consequences for the individual and society. Recent results from the National Health and Nutrition Examination Survey indicate that over 33% of children between the ages of 2 to 19 years are either at risk for overweight or overweight. The aim of the study was to determine the effects of obesity on self-concept, depression and quality of life among adolescents. A descriptive correlational design was utilized in the current study. The current study was carried at the out- patient clinic of endocrinology, El-Monera Pediatric Hospital. One hundred obese adolescents [according to BMI] were selected in the study. Four tools were used: Socio-demographic data sheet, Pediatric Self Concept Scale, Pediatric Quality of Life Inventory [Peds QL], and Children's Depression Inventory [CDI]. Seventy five percent of the sample were 10-12 years old, and minority of the studied sample suffer from high depressive manifestations. The study concluded that, there are a statistical significant relationship between total scores of Pediatric QOL scale and both of total scores of CDI, and self concept. Also between total scores of CDI and total scores of self- concept. The main problem could be related not only to being obese, but also to the perception of having a higher than ideal weight. So it is essential to evaluate weight perception in addition to body mass index [BMI]. Also, health care providers should focus on modest weight - loss goals that correlate with significant health benefits. The most effective treatments include substantial parental involvement

[Studies on transformation of resistant plasmid between salmonella and e. col1]

Plasmid profile analysis of 10 multiple drug resistant Salmonella serovars with [resistant to am-picillin, chloramphenicol, oxytetracycline, nali-dixic acid and streptomycin] was performed using agarose gel electrophoresis. There were 8 isolates harbouring plasmid with molecular weight ranged from 16 - 31.5 kbp while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S. Kentucky and S. Typhirnurium as donor bacteria and E. coli XL1 as recipient bacteria by electroporation. Transconjugants were obtained by conjugation assay between multiple drug resistant [MDR] S. Typhimurium as donor bacteria and each of S. Enteritidis , S. Typhi, E. coli: XL1 and E. coli: O157H7 as recipient bacteria?

Effect of preventive guidelines for women with risk factors of breast cancer

Breast cancer represents among females, the most important cancer sites in term of incidence and mortality. The objective of this study was to assess women attending to the Gynecology Clinic, Breast Clinic and Mammogram Unit for risk factors of breast cancer for early detection, and counseling. A guideline booklet about preventive measures of breast cancer was developed to evaluate its effect on women's behavior. The research questions were: [1] what are the most common risk factors among the Egyptian women? [2] what is the effect of the health guideline booklet on women's behavior? The total sample was 470 Egyptian women selected randomly from those who, attended the clinics from 15 September 2003 to 15 February 2004. The women with risk factors of breast cancer were 210 only. They were provided with a health guideline booklet. Their mean age was 35.5 +/- 17.1. Most risk factors appeared among the high risk group of women using hormonal contraceptives for more than 10 years [48.7%]. The study recommended wide guidelines for improving women's behaviors towards the preventive measures and early detection of breast cancer through the proper management. Continued efforts are needed to increase the availability of high quality mammography and treatment to all segments of the population. This study concluded that the Egyptian women had a positive readiness to change their behavior through more awareness about risk factors and preventive measures of breast cancer

effect of palm date intake during prelabour period on labour outcome among high risky women

Labour and birth are a natural process, they usually progress normally with no problems for mother and baby. Palm date or phoenix dactyl feral [tamr] is the most important source of energy. The present study aimed to investigate the effect of palm date [tamr] intake during the prelabour [two weeks] period on labour outcome among high risky women. This study included two groups: First group [experimental group 60] primigravida women who received antenatal and natal care and were eating for two weeks extra meal palm dates. Second group: [control group 70] primigravida women who received only a routine antenatal and natal care. Three tools were used for collecting data [sheet of general characteristics, Bishop score and partogram chart], which started from January to June 2005 [6 months], women constituted. Finding showed that the means of Bishop Scores of the study sample [experimental and control groups] at tune of their admission for labour were 10.5 +/- 1.1 and 7.2 +/- 1.1 respectively [t = 3.9] with significant difference P<0.05 between the two groups. The means and the S.D of the rate of cervical dilatation during active phase of labour in cm/hours among two groups [experimental and control] were 1.3 +/- 0.3 and 1 +/- 0.1 [t= 3.7]. Mean duration of labour of the two groups [experimental and control] were 5.1 +/- 0.9 and 6.5 +/- 1 respectively [t= 4.1]. The largest percent of the experimental group delivered spontaneous [88.3%] compared to [58.6%] in the control group. One minute Apgar score was >7 for most of the studied women's babies in both groups [90% and 86%]. Results revealed that [51.4%] of the control group need oxytocin augmentation compared to only [8.3%] in the experimental. There were statistically significant differences between the two groups [experimental and control] to the effect of taking palm date [tamr] during the prelabour [two weeks] period on labour outcome of the experimental group, mode and duration of delivery as well as the Apgar score of the newborn. The study recommended that materials as booklets should be available and provided to pregnant women about the importance of palm date intake during a prelabour period. A national program about health nutrition for pregnant women to facilitate labour activity should be provided