RESUMEN
The use of stem cells in regenerative medicine had great potential for clinical applications. However, cell delivery strategies have critical importance in stimulating the differentiation of stem cells and enhancing their potential to regenerate damaged tissues. Different strategies have been used to investigate the osteogenic potential of dental stem cells in conjunction with biomaterials through in vitro and in vivo studies. Osteogenesis has a broad implication in regenerative medicine, particularly for maxillofacial defects. This review summarizes some of the most recent developments in the field of tissue engineering using dental stem cells.
RESUMEN
Background & objectives: Platelet concentrate contains a rich cocktail of growth factors that support growth and proliferation of cells. The primary goal of this study was to investigate the safety of platelet lysate (PL) in patients with critical limb ischaemia (CLI) not suitable for standard revascularization. Data on the preliminary efficacy are also presented. Methods: Seven patients (18-70 yr) with CLI classified in the Rutherford grades 3-5, with history of intermittent claudication for more than eight weeks and were not suitable for standard revascularization, underwent autologous intramuscular injections of PL. These patients were examined physically alongside other parameters such as TcPO2, toe pressure, and ankle brachial index, at baseline and were followed up for 12 months. Results: The procedure was well tolerated with no serious adverse or any adverse events reported during follow up. Although not the primary aim of this report, preliminary data showed significant clinical improvement in Rutherford stage, ankle-brachial index and toe pressure which persisted for a year. Interpretation & conclusions: Intramuscular injection of PL was well tolerated with no major adverse events reported in our study participants. With the observed satisfactory safety data, preliminary efficacy data of PL should be further validated.
RESUMEN
Probiotics, live cells having various health-promoting effects and benefits to the host, have been, and still are, under extensive investigations. These "friendly microorganisms" are usually isolated from the human intestine and traditionally-fermented foods of plant and animal origin. They are recognized for their positive effects on the intestinal flora among many other direct and indirect benefits eliciting better human body functioning. Studies are being conducted in the last decade o substantiate the real role played by these exotic microorganisms and to ascertain the safety of its use being of probiotic potential. The concept of disease prevention, rather than treatment, is most appealing to health providers and clinical settings world-wide. Dentistry is not an exception; preventive oral medicine is a significant pillar in modern dental practices. In fact, a large proportion of every day dental care, in addition to clinical treatments, aims at preventing oral diseases, including caries, gingivitis, periodontitis and halitosis. Based on varying results of so many clinical trials using different types and formulas of experimental oral probiotics and to shed some light on the questionable role and effectiveness of probiotics on the prevention and treatment of oral diseases, this mini review is presented. We believe that exploring the possible mechanisms of action of probiotic bacteria in the oral cavity and identifying specific potential uses of probiotics in preventive dentistry and stomatology is eminent and needs further investigations pertaining to developing a safe, efficient and reliable general and perhaps designer oral probiotics