Effects of huperzine A on cognitive function of rats recovering from general anesthesia / 南方医科大学学报

<p><b>OBJECTIVE</b>To observe the effect of huperzine A on the cognitive function of rats recovering from general anesthesia and discuss its possible mechanism.</p><p><b>METHODS</b>Sixty rats (20 to 23 weeks old) were subjected to spatial reference memory version of navigation task, in which the rats were expected to locate the escape platform in water. Two sessions of training were given daily for 5 days, and on the 5th day, the escape latencies of the rats were recorded. The rats were then divided randomly into 5 groups (n=12), and in 4 of the groups, the rats received an intraperitoneal injection of diprivan (Dip) at 2 mg/kg and after recovery of righting reflex, huperzine A was given at 0.05 mg/kg (group L), 0.1 mg/kg (group M), 0.2 mg/kg (group H), and in group C, no subsequent huperzine A was given; in group E, the rats received normal saline injection only. One hour after righting reflex recovery, the escape latencies of all the rats were recorded again, and the level of AChE expression in the forebrain cortex was measured quantitatively.</p><p><b>RESULTS</b>The escape latencies after righting reflex recovery was significantly longer than that on day 5 (P<0.05), and the rats in group H had the shortest escape latency among the groups (P<0.05). The average gray scale of AChE in the forebrain of rats in group H was significantly lower than that of the other groups (P<0.05).</p><p><b>CONCLUSION</b>Huperzine A can inhibit cholinesterase in the brain to improve the cognitive function of rats recovering from general anesthesia.</p>

Relationship between apolipoprotein e4 allele and emergence agitation in patients undergoing general anesthesia / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the relationship between apolipoprotein e4 allele and emergence agitation (EA) in patients undergoing general anesthesia.</p><p><b>METHODS</b>A nested cohort study was conducted in elderly patients (over 60 years old) scheduled for major abdominal surgery requiring general anesthesia. A structured interview was conducted in PACU to determine EA, defined using the Sedation-Agitation Scale (SAS). Blood samples were obtained for measurement of the apolipoprotein genotypes.</p><p><b>RESULTS</b>Of the 196 patients studied, 22.4% developed EA. Thirty-eight patients (19.4%) had the apolipoprotein e4 allele. The presence of the e4 allele and low level of education were both associated with an increased risk of EA (36.9% vs15.8%, P=0.005; 30% vs 14.3%, P=0.01). After adjustment for covariates, the patients with the copy of e4 allele were shown to have a greater likeliness of an increased risk of EA (odds ratio: 4.32; 95% CI: 1.75-10.05) than those without the e4 allele.</p><p><b>CONCLUSION</b>Apolipoprotein e4 carrier status is associated with an increased risk for EA.</p>