Biochemical and bacteriological study on renal stone

Results of studying 203 calculi taken from 203 patients showed that the males are more affected than females with a proportion of 1.7:1. The age group [40 - 49 yrs] was the most affected followed by age group [50-59 yrs]. Calcium calculi made the greatest constitution of calculi in proportion of 89.63% of the renal stones. Six bacterial species were isolated from culture of 203 stones comprised the following: Proteus mirabilis, Escherichia coli, Staphylococciis aureus, Psendomonas aeruginosa, Klebsiella pneumonia, and Citrobacter freundii. However, P.aeruginosa made the greatest proportion about 67.97% of the total stones number, while 40 stones did not yield a growth of any bacteria. Even though external layers and nidi of the stones showed the same bacterial species, culture of pelvis urine did not yield bacterial growth.

Role of bacteria in Urolithiasis [in vivo study]

The different bacterial species that were isolated from human renal stones: Pseudomonas aerugniosa [urease +ve], Proteus mirabilis, Citrohacler freundii, Klebsiella pneumonia, Staphylococcus aureus, Pseudomonas aeruginosa, [urease -ve], and Escherichia coli, succeeded in stone formation in rats' kidneys, this was determined by examining tissue sections of the kidneys. However, urease producing bacteria revealed more severe than non-urease ones, which influenced, markedly, in increasing urine volume after 10 days of bacterial injecting followed by a decline after 20 - 30 days, however, pH was acidic during the first ten days of injection, changed to alkalinity later, whereas those had been injected with non-urease producers, did not show such changes in their urine.