RESUMEN
Background and Aims: type and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by humans have different compositions. One of the important apolipoproteins is apolipoprotein B [apoB]. This study was conducted to compare the effect of different nutritious fats on expression of apoB protein
Materials and Methods: for this purpose, 48 Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with 10% corn oil, diet with 10% olive oil, diet with 10% cow ghee, diet with 10% sheep ghee, and diet with 2% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen at -70degreeC. Protein was extracted and the expression of apoB was studied by western blotting
Results: an increase in the expression of intestinal apoB48 was identified in olive oil and cow ghee groups. Hepatic apoB100 expression was increased in the cholesterol group compared with the corn oil group
Conclusion: this study indicates that olive oil and cow ghee consumption increase intestinal apoB48 expression