Burn scar reconstruction of the neck with FTSG obtained from lower abdominal skin

Burns account for a significant proportion of injuries, and of these the face, neck, and anterior torso are commonly affected. Burn scars remain a lasting reminder of the insult both for the patient and the outside world. There is little doubt that the change in appearance and the limitation imposed by a burn scar contribute to negative body image. Treatment of hypertrophic scars in the neck has been quite challenging if there is no intact tissue for local flaps. So application of full-thickness skin grafts could be of great value. We applied full-thickness grafts obtained from lower abdominal skin for treatment of severe neck contractures in four patients when other treatment modalities such as local flaps could not be used. Full-thickness skin graft of the neck is a safe and reliable treatment option with fairly good functional and aesthetic results. It has little donor site morbidity in spite of providing a large surface area of full-thickness skin

Evaluation of different sperm immunization methods in mice

Background: Antifertility effect of naturally occuring antisperm antibody [ASA] in infertile couples and studies on experimental immunization of various animals with sperm antigens represents ASA as immunocontraceptive target. Despite extensive research on the effects of different factors on sperm immunogenecity and ASA production variable result have been reported

Objective: To study whole sperm immunization in mice

Methods: In an experimental study, whole mice sperm with different adjuvant i.e. complete Freund's adjuvant [CFA], incomplete Freund's adjuvant [ICFA], and cholera toxin subunit-p [CTS-[3] were administrated to mice intramuscularly [IM], subcutaneously [SC], intranasally [IN], intra-peritoneally [IP], intrarectally [IR], intravaginally [IVA] and orally. Control groups were inoculated with phosphate buffer saline [PBS] plus corresponding adjuvant. Immunization was carried out on days 0, 7, 14, 28 and ASA titers were detected by indirect immunoflu-orescence [IFA] technique in sera and vaginal washes of all groups. The IP group was further excluded from the study due to high mortality rate. The results were compared between control and experimental groups by Mann Whitney and Fisher exact tests

Results: The number of positive mice for ASA in IM, SC, IN experimental and control groups were significantly different [P = 0.01, P = 0.01, P = 0.04, respectively]. However, there were no significant differences between IR, IVA, and oral experimental and control groups. No differences were observed between ASA in vaginal washing of all groups. Due to high mortality in IP group it was excluded from the study

Conclusion: It can be concluded that the whole sperm antigen can induce immune response in female mice by IM, SC, IN but not IAV, IR and oral administration routes