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1.
National Journal of Andrology ; (12): 41-44, 2009.
Artículo en Chino | WPRIM | ID: wpr-292426

RESUMEN

<p><b>OBJECTIVE</b>To observe the expression of Smad4, the core of TGF-beta/Smads signal transduction pathway in different prostate cancer cell lines, and explore their molecular mechanism of bone metastatic potential.</p><p><b>METHODS</b>The Millicell polycarbonate filter coated with matrigel was used to confirm the invasive potency of LNCaP and ARCaP cell lines (IF11 and IA8). The expressions of the Smad4 protein and mRNA in these prostate cancer cells with different metastatic potentials were detected by Western blotting and RT-PCR, respectively.</p><p><b>RESULTS</b>ARCaP cell lines (IF11 and IA8) exhibited a stronger potency of invasion than LNCaP (P < 0.01). The Smad4 protein and mRNA highly expressed in the LNCaP cell line that was well-known with a low metastatic potential, but not in the ARCaP (IF11 or IA8) cells with high metastatic potentials (P < 0.01).</p><p><b>CONCLUSION</b>Smad4 expresses differently in LNCaP and ARCaP cell lines with different metastatic potentials and, as a tumor suppressive gene, its deficient expression may play an important role in the invasion and metastasis of advanced prostate cancer.</p>


Asunto(s)
Humanos , Masculino , Línea Celular Tumoral , Metástasis de la Neoplasia , Neoplasias de la Próstata , Metabolismo , Patología , ARN Mensajero , Genética , Proteína Smad4 , Factor de Crecimiento Transformador beta , Metabolismo
2.
National Journal of Andrology ; (12): 238-241, 2008.
Artículo en Chino | WPRIM | ID: wpr-319237

RESUMEN

<p><b>OBJECTIVE</b>To determine the effect of the transforming growth factor beta (TGF-beta) on the expression of invasion and metastasis associated proteins in the prostate cancer LNCaP cell line in vitro.</p><p><b>METHODS</b>The prostate cancer cell line LNCaP was treated with TGF-beta in vitro. Western blotting was used to detect the expression of the "invasion and metastasis" associated proteins E-Cadherin, N-Cadherin and Vimentin.</p><p><b>RESULTS</b>The expression of N-Cadherin and Vimentin of the LNCaP cells treated with TGF-beta for 12 hours was significantly upregulated, but not that of E-Cadherin.</p><p><b>CONCLUSION</b>TGF-beta may induce epithelial-mesenchymal transition (EMT) of LNCaP cells which might be of importance in promoting prostate cancer cells invading to ambient tissues and metastasizing to distant organs.</p>


Asunto(s)
Humanos , Masculino , Western Blotting , Cadherinas , Línea Celular Tumoral , Neoplasias de la Próstata , Metabolismo , Patología , Factor de Crecimiento Transformador beta , Farmacología , Regulación hacia Arriba , Vimentina
3.
National Journal of Andrology ; (12): 439-444, 2008.
Artículo en Chino | WPRIM | ID: wpr-319216

RESUMEN

<p><b>OBJECTIVE</b>To observe the influence of hypoxia-inducible factor 1 alpha (HIF-1alpha) on angiogenesis in prostate carcinoma in vivo and to investigate its molecular mechanism.</p><p><b>METHODS</b>LNCaP/HIF-1alpha and LNCaP cells were cultured, the level of PSA in the supernatant of the culture medium detected by ELISA assay before and after the transfection, and the cellular cycle measured by flow cytometry. Nude mouse models of subcutaneous tumor were established with LNCaP/HIF-1alpha and LNCaP cells, the tumor growth observed, and tumor specimens collected for immunohistochemical staining.</p><p><b>RESULTS</b>Compared with the LNCaP cells, LNCaP/HIF-1alpha cells showed an obviously decreased PSA level (t = 8.243, P < 0.05) and enhanced proliferous activity. The tumorigenesis rate increased and the tumorigenesis time advanced in the LNCaP/HIF-1alpha group of the nude mice. Immunohistochemistry displayed higher expressions of VEGF, iNOS and Ang-2 in the LNCaP/HIF-1alpha than in the LNCaP group.</p><p><b>CONCLUSION</b>The over-expression of HIF-1alpha can up-regulate VEGF and iNOS involved in angiogenesis in vivo and contribute to the invasive potency of LNCaP cells. HIF-1alpha may have no influence on Ang-2 either in vitro or in vivo, while the expression of Ang-2 is regulated by other factors in vivo.</p>


Asunto(s)
Animales , Humanos , Masculino , Ratones , Ciclo Celular , Línea Celular Tumoral , Proliferación Celular , Citometría de Flujo , Regulación Neoplásica de la Expresión Génica , Subunidad alfa del Factor 1 Inducible por Hipoxia , Genética , Fisiología , Inmunohistoquímica , Ratones Desnudos , Neoplasias Experimentales , Metabolismo , Patología , Neovascularización Patológica , Óxido Nítrico Sintasa de Tipo II , Neoplasias de la Próstata , Genética , Patología , Transfección , Trasplante Heterólogo , Carga Tumoral , Factor A de Crecimiento Endotelial Vascular
4.
National Journal of Andrology ; (12): 988-991, 2007.
Artículo en Chino | WPRIM | ID: wpr-232026

RESUMEN

<p><b>OBJECTIVE</b>To evaluate the effect of hypoxia-inducible factor-1 alpha (HIF-la) on angiogenesis in human prostate cancer cells.</p><p><b>METHODS</b>Human prostate cancer cells of the line LNCaP were cultured and transfected by the recombinant plasmid pcDNA3. 1(-)/HIF-1alpha containing the gene HIF-1alpha with the Lipofectamine 2000 system. The positive clone cells were selected by G418 and confirmed by Western blotting and immunofluorescence (LNCaP/HIF-1alpha cells). The expressions of VEGF, iNOS and Ang-2 were detected by Western blotting.</p><p><b>RESULTS</b>The expression of HIF-1alpha in the LNCaP/HIF-1alpha cells was distinctly higher than that in the LN-CaP cells. Compared with the LNCaP cells, the expressions of VEGF and iNOS were up-regulated, whereas Ang-2 remained unchanged in the LNCaP/HIF-1alpha cells.</p><p><b>CONCLUSION</b>The over-expression of HIF-1alpha can induce an increase in angiogenesis proteins and improve the angiogenesis potency of prostate cancer.</p>


Asunto(s)
Humanos , Masculino , Western Blotting , Línea Celular Tumoral , Técnica del Anticuerpo Fluorescente , Subunidad alfa del Factor 1 Inducible por Hipoxia , Genética , Metabolismo , Lípidos , Química , Óxido Nítrico Sintasa , Metabolismo , Plásmidos , Química , Genética , Neoplasias de la Próstata , Metabolismo , Patología , Transfección , Métodos , Factor A de Crecimiento Endotelial Vascular , Metabolismo
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