Expression of NLRP3 inflammasome in BALB/c mice with imiquimod-induced psoriasis-like inflammation and therapeutic effect of mustard seed (Sinapis Alba Linn) / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the role of NLRP3 inflammasome in imiquimod-induced psoriasis-like inflammation in mice and the therapeutic effects of mustard seed (Sinapis Alba Linn).</p><p><b>METHODS</b>Thirty BALB/c mice were randomized equally into blank control group (fed with normal forage and treated with vehicle), model group (fed with normal forage and treated with 5% imiquimod cream), and experimental group (fed with 5% mustard seed forage and treated with 5% imiquimod cream). RT-PCR was used to detect the mRNA expression of NLRP3, ASC, caspase-1, and caspase-11. Immunohistochemistry was performed to determine the expression and distribution of ASC and caspase-1. ELISA was used to test the serum levels of interleukin-1β (IL-1β) and IL-18.</p><p><b>RESULTS</b>Compared with the blank control group, the mice with imiquimod-induced psoriasis-like inflammation showed significantly increased NLRP3, ASC, caspase-1, and caspase-11 mRNA expressions, ASC and caspase-1 protein expressions , and serum levels of IL-1β and IL-18 (P<0.05). These changes were obviously attenuated by feeding the mice with mustard seed.</p><p><b>CONCLUSION</b>NLRP3 inflammasome is involved in imiquimod-induced psoriasis-like inflammation in mice, and mustard seed may suppress the inflammation induced by IL-1β and IL-18 through down-regulating the expression of NLRP3 inflammasome.</p>

Effects of mustard seed on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the therapeutic effect of mustard seed on allergic contact dermatitis (ACD) in mice and explore the mechanism.</p><p><b>METHODS</b>Eighteen BALB/c mice were randomly divided into normal control group, model group and mustard seed group. The mice in the normal control group and model group were fed with normal chow, and those in mustard seed group were given 5% mustard seed mixed in the chow. Three weeks later, ACD was induced on the ear using 2, 4-dinitrofluorobenzene. After 24 h, the swelling of the ear was examined, and the rats were sacrificed to collect the ear tissue ears and blood for histopathological and immunohistochemical examinations, RT-PCR and enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>In mice with ACD, feeding with mustard seeds significantly lessened the ear swelling, improved the tissue histopathology, lowered the number of infiltrating Langerhans cells, and reduced the expressions of IL-1β, TNF-α and IL-6 mRNA in the ear, but did not cause significant changes in serum levels of IL-4, IFN-γ and IL-17.</p><p><b>CONCLUSION</b>Mustard seed inhibits ACD in mice possibly by suppressing the expressions of IL-1β, TNF-α and IL-6 mRNA and inhibiting Langerhans cell migration in the epidermis.</p>

Clinical efficacy of Qingpeng ointment for the treatment of chronic eczema / 中华皮肤科杂志

ObjectiveTo evaluate the efficacy and safety of Qingpeng ointment in the treatment of chronic eczema.MethodsTotally,95 patients with chronic eczema were enrolled and divided into 3 groups to receive topical Qingpeng ointment (n =33),0.2％ hydrocortisone cream (n =32),10％ urea ointment (n =30) respectively twice a day for 4 weeks.All the patients were followed up once a week for the evaluation of therapeutic and side effects.ResultsAfter 4-week treatment,the response rate was 78.8％,84.4％ and 46.7％ in the Qingpeng ointment-,hydrocortisone cream- and urea ointment-treated patients respectively.Significant differences were observed in the response rate between the Qingpeng ointment- and urea ointment-treated patients (P ＜ 0.05 ),but not between the hydrocortisone cream- and Qingpeng ointment-treated patients (P ＞0.05).Skin flushing with a sensation of burning was observed at the application sites of 2 patients treated with Qingpeng ointment,and tingling sensation at the application sites was reported in 1 patient treated with hydrocortisone cream.All of these adverse reactions were tolerable and had no influence on the treatment.Conclusion Topical Qingpeng ointment is effective and safe for the treatment of chronic eczema.

Expression and significance of cannabinoid receptor 2 in malignant melanoma / 中华皮肤科杂志

Objective To investigate the expression of cannabinoid receptor 2 (CB2) in pigmented nevus and malignant melanoma and its significance. Methods The protein and mRNA expressions of CB2 were measured in tissue samples of 20 patients with pigmented nevus and 20 patients with malignant melanoma using immunohistochemical staining and RT-PCR, respectively. Results CB2 was expressed in both pigmented nevus and malignant melanoma. In tissue of pigmented nevus, CB2 was chiefly expressed in nevocytes and basal cell layer, and only a small quantity of CB2 was expressed in subcutaneous tissue. Statis-tical differences were observed between samples of malignant melanoma and pigmented nevus in the expres-sion intensity of CB2 (P < 0.05). Conclusions Increased protein and mRNA expressions of CB2 are observed in tissue of malignant melanoma, and the expression intensity of CB2 is higher in tissue of malig-nant melanoma than in that of pigmented nevus.

Two Methods of Identification for Trichophyton rubrum / 中华医院感染学杂志

OBJECTIVE To compare modified Kane/Fischer system and PCR method in identification for Trichophyton rubrum.METHODS Using modified Kane/Fischer system and TR1F and TR1R primers to identify 32 strains of dermatophytes.RESULTS The modified Kane/Fischer system and PCR could rapidly and stably(identify) T.rubrum.CONCLUSIONS The two methods can be used as the standard system to identify T.rubrum.

Localization of dopamine- and cAMP-regulated phosphoprotein in mouse kidney / 第三军医大学学报

Objective To investigate the expression and localization of dopamine- and cAMP-regulated phosphoprotein of 32 000 (DARPP-32) in mouse kidney tissue. Methods The cellular localization of DARPP-32 in mouse kidney tissue was detected by immunoblot and immunohistochemistry. Results DARPP-32-like immunoreactivity was detected in the medullary thick ascending limb of the loop of Henle, the cortical proximal convoluted tubule, and collecting ducts in medullary rays. The renal tubules were enriched of Na+, K+-ATPase for sodium reabsorption. Conclusion The participation of DARPP-32 is a likely crucial step of the signal-transduction pathway of dopamine regulation on sodium reabsorption in renal tubule cells.

Therapeutic Effect of Compound Glycyrrhizin in the Treatment of Acute Urticaria / 中国药房

0.05);while great significances were found between group C and group B and A(P

Psoriasis vulgaris and Helicobacter pylori infection / 解放军医学杂志

Objective To explore the relationship between Helicobacter pylori (Hp) infection and psoriasis vulgaris, and investigate the effect of anti-Hp therapy on the patients with psoriasis vulgaris. Methods 13C-urea expiration test and detection of Hp antigen in feces samples were performed to identify Hp infection in 62 psoriasis patients and 35 healthy persons. Detection results of the two groups were statistically analyzed. The 62 psoriasis patients were divided into two groups based on the detection of Hp infection: Hp positive group (32 cases) and Hp negative group (30 cases). Patients in Hp positive group were given anti-Hp triple therapy (omeprazole, amoxicillin, clarithromycin) for two weeks and topical application of tretinoin for six weeks, while the patients in Hp negative group were treated only with topical use of tretinoin for six weeks. The total effective rate, score of psoriasis area, severity index (PASI) and visual analogue scale (VAS) of pruritus were recorded and then analyzed. Results The Hp infection rate in 62 psoriasis patients was 51.6%, and in healthy individuals was 22.9%, the difference between the two groups was statistically significant (P

Expression and localization of CB2 (cannabinoid receptor) in rat skin / 解放军医学杂志

Objective To study the expression and distribution of CB2 in rat skin tissue. Method Immunohistochemistrial technics were employed to study the expression and distribution of CB2 in rat skin tissue. Results CB 2 receptors mostly presented in suprabasal layers of the epidermis and hair follicles in dermis by CB2 like immunoreactivity with a N terminal anti CB2 receptor antibody. The positive expression of CB2 appeared in rat spleen and a negative result occured in liver. Conclusion The CB2 receptors distributed mainly in the rats, epidermis and hair follicles, may involve in some of the dermic physiological and pathological process, such as the communication between central neural system and skin.