RESUMEN
Objective To establish a method for determination of puerarin in Yangwei Soft Capsule. Methods HPLC was used for quantitative ananlysis. The Phenomenex Kromasil C18 (250 mm?4.6 mm,5 ?m) column was used,and mobile phase was composed of methanol-water (22∶78). Detection wavelength was at 294 nm,the flow rate was 1.0 mL/min,column temperature was at 40 ℃,the injection volume was 10 ?L. Results The linear response ranges from 0.058 8～0.882 0 ?g of magnolol (r=0.999 9,n=6). The average recovery of magnolol is 99.35%,RSD=1.94%. The linear response ranges from 0.020 4～0.306 0 ?g of honokio (r =0.999 8,n =6). The averagy recovery of honokio is 99.21%,RSD=2.10%. Conclusion The method is simple,accurate,and suitable for the determination of the purarin in compound preparations.