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J Genet ; 2002 Apr; 81(1): 5-11
Artículo en Inglés | IMSEAR | ID: sea-114312

RESUMEN

A sporulating culture of Bacillus thuringiensis subsp. kenyae strain HD549 is toxic to larvae of lepidopteran insect species such as Spodoptera litura, Helicoverpa armigera and Phthorimaea operculella, and a dipteran insect, Culex fatigans. A 1.9-kb DNA fragment, PCR-amplified from HD549 using cryII-gene-specific primers, was cloned and expressed in E. coli. The recombinant protein produced 92% mortality in first-instar larvae of Spodoptera litura and 86% inhibition of adult emergence in Phthorimaea operculella, but showed very low toxicity against Helicoverpa armigera, and lower mortality against third-instar larvae of dipteran insects Culex fatigans, Anopheles stephensi and Aedes aegypti. The sequence of the cloned crystal protein gene showed almost complete homology with a mosquitocidal toxin gene from Bacillus thuringiensis var. kurstaki, with only five mutations scattered in different regions. Amino acid alignment with different insecticidal crystal proteins using the MUTALIN program suggested presence of the conserved block 3 region in the sequence of this protein. A mutation in codon 409 of this gene that changes a highly conserved phenylalanine residue to serine lies in this block.


Asunto(s)
Secuencia de Aminoácidos , Animales , Bacillus thuringiensis/genética , Proteínas Bacterianas/genética , Toxinas Bacterianas , Clonación Molecular , ADN Bacteriano/genética , Endotoxinas/genética , Genes Bacterianos , Proteínas Hemolisinas , Insectos , Datos de Secuencia Molecular , Proteínas Recombinantes/genética , Análisis de Secuencia de ADN , Homología de Secuencia de Aminoácido
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