Treatment after bleaching for optimal bonding

No abstract available.

Treatment after bleaching for optimal bonding

No abstract available.

Immunogenicity of a Canine Parvovirus 2 Capsid Antigen (VP2-S1) surface-expressed on Lactobacillus casei / 동물의과학연구지

Canine parvovirus (CPV2) is one of the most virulent virus causing acute hemorrhagic enteritis and myocarditis in dogs. Infection mainly caused by the ingestion of virus through the mucosal route. Therefore, induction of mucosal immunity is essential in prevention of Canine Parvovirus (CPV2) infection. For safe and effective delivery of viral antigens to the mucosal immune system, a novel surface antigen display system for lactic acid bacteria using the poly-gamma-glutamic acid synthetase A protein (pgsA) of Bacillus subtilis as an anchoring matrix was applied in order to display CPV2 antigen on the surface of the recombinant L. casei. Recombinant fusion proteins comprised of pgsA and the capsid protein (VP2-S1) showed stable expression in Lactobacillus casei. Surface localization of the fusion protein was verified by cellular fractionation analyses. Oral and nasal inoculations of recombinant L. casei into mice resulted in high levels of serum immunoglobulin G (IgG) and mucosal IgA, as demonstrated by ELISA using recombinant VP2-S1 proteins. Mice receiving intranasal immunization mounted higher antibody response than those receiving oral immunization. These results indicate that mucosal immunization with recombinant L. casei expressing CPV2 VP2-S1 protein on its surface provides an effective means for elicitation of strong antibody responses against CPV 2 VP2-S1.

Effect of different chlorhexidine application times on microtensile bond strength to dentin in Class I cavities

OBJECTIVES: This study evaluated the effect of 2% chlorhexidine digluconate (CHX) with different application times on microtensile bonds strength (MTBS) to dentin in class I cavities and intended to search for ideal application time for a simplified bonding protocol. MATERIALS AND METHODS: Flat dentinal surfaces with class I cavities (4 mm x 4 mm x 2 mm) in 40 molar teeth were bonded with etch-and-rinse adhesive system, Adper Single Bond 2 (3M ESPE) after: (1) etching only as a control group; (2) etching + CHX 5 sec + rinsing; (3) etching + CHX 15 sec + rinsing; (4) etching + CHX 30 sec + rinsing; and (5) etching + CHX 60 sec + rinsing. Resin composite was built-up with Z-250 (3M ESPE) using a bulk method and polymerized for 40 sec. For each condition, half of the specimens were immediately submitted to MTBS test and the rest of them were assigned to thermocycling of 10,000 cycles between 5degrees C and 55degrees C before testing. The data were analyzed using two-way ANOVA, at a significance level of 95%. RESULTS: There was no significant difference in bond strength between CHX pre-treated group and control group at the immediate testing period. After thermocycling, all groups showed reduced bond strength irrespective of the CHX use. However, groups treated with CHX maintained significantly higher MTBS than control group (p < 0.05). In addition, CHX application time did not have any significant influence on the bond strength among groups treated with CHX. CONCLUSION: Application of 2% CHX for a short time period (5 sec) after etching with 37% phosphoric acid may be sufficient to preserve dentin bond strength.

Effects of canal enlargement and irrigation needle depth on the cleaning of the root canal system at 3 mm from the apex

OBJECTIVES: The aim of this study was to test the hypothesis, that the effectiveness of irrigation in removing smear layer in the apical third of root canal system is dependent on the depth of placement of the irrigation needle into the root canal and the enlargement size of the canal. MATERIALS AND METHODS: Eighty sound human lower incisors were divided into eight groups according to the enlargement size (#25, #30, #35 and #40) and the needle penetration depth (3 mm from working length, WL-3 mm and 9 mm from working length, WL-9 mm). Each canal was enlarged to working length with Profile.06 Rotary Ni-Ti files and irrigated with 5.25% NaOCl. Then, each canal received a final irrigation with 3 mL of 3% EDTA for 4 min, followed by 5 mL of 5.25% NaOCl at different level (WL-3 mm and WL-9 mm) from working length. Each specimen was prepared for the scanning electron microscope (SEM). Photographs of the 3mm area from the apical constriction of each canal with a magnification of x250, x500, x1,000, x2,500 were taken for the final evaluation. RESULTS: Removal of smear layer in WL-3 mm group showed a significantly different effect when the canal was enlarged to larger than #30. There was a significant difference in removing apical smear layer between the needle penetration depth of WL-3 mm and WL-9 mm. CONCLUSIONS: Removal of smear layer from the apical portion of root canals was effectively accomplished with apical instrumentation to #35/40 06 taper file and 3 mm needle penetration from the working length.

An embouchure aid for saxophone player

This study aims to introduce the method that can relieve vibrating forces to oral environment by making an embouchure aid. Thin plastic crown forms were fabricated to prevent tooth abrasion and irritation to lip mucosa for the saxophone player. After application to the player, the most comfort form was chosen and delivered to 3 professional saxophone players. After 5 mon, the players responded to the survey. This embouchure aid did not disturb playing and gave comfort to lower lip. In general, the players preferred thin soft type and thought it caused little effect on sound. Far too little attention has been paid to the problems encountered by single-reed wind instrumentalist who suffer from tooth abrasion and irritation to lip mucosa. The embouchure aid not only prevent tooth damage but also diminish the discomfort of tight embouchure.

Effect of CQ-amine ratio on the degree of conversion in resin monomers with binary and ternary photoinitiation systems

OBJECTIVES: This study evaluated the effect of camphorquinone (CQ)-amine ratio on the C=C double bond conversion of resins with binary and ternary photoinitiation systems. MATERIALS AND METHODS: Two monomer mixtures (37.5 Bis-GMA/37.5 Bis-EMA/25 TEGDMA) with binary systems (CQ/DMAEMA in weight ratio, group A [0.5/1.0] and B [1.0/0.5]) and four mixtures with ternary system (CQ/OPPI/DMAEMA, group C [0.1/1.0/0.1], D [0.1/1.0/0.2], E [0.2/1.0/0.1] and F [0.2/1.0/0.2]) were tested: 1 : 2 or 2 : 1 CQ-amine ratio in binary system, while 1 : 1 ratio was added in ternary system. The monomer mixture was cured for 5, 20, 40, and 300 sec with a Demetron 400 curing unit (Demetron). After each exposure time, degree of conversion (DC) was estimated using Fourier transform infrared (FTIR) spectrophotometer (Nicolet 520, Nicolet Instrument Corp.). The results were analyzed by ANOVA followed by Scheffe test, with p = 0.05 as the level of significance. RESULTS: DC (%) was expressed in the order of curing time (5, 20, 40, and 300 sec). Group A (14.63 +/- 10.42, 25.23 +/- 6.32, 51.62 +/- 2.69, 68.52 +/- 2.77); Group B (4.04 +/- 6.23, 16.56 +/- 3.38, 37.95 +/- 2.79, 64.48 +/- 1.21); Group C (16.87 +/- 5.72, 55.47 +/- 2.75, 60.83 +/- 2.07, 68.32 +/- 3.31); Group D (23.77 +/- 1.64, 61.05 +/- 1.82, 65.13 +/- 2.09, 71.87 +/- 1.17); Group E (28.66 +/- 2.92, 56.68 +/- 1.33, 60.66 +/- 1.17, 68.78 +/- 1.30); Group F (39.74 +/- 6.31, 61.07 +/- 2.58, 64.22 +/- 2.29, 69.94 +/- 2.15). CONCLUSION: All the monomers with ternary photoinitiation system showed higher DC than the ones with binary system, until 40 sec. Concerning about the effect of CQ-amine ratio on the DC, group A converted into polymer more than group B in binary system. However, there was no significant difference among groups with ternary system, except group C when cured for 5 sec only.

Erratum: Correction of inverted Figures in Table 1

The figures in Table 1 were inverted upside down in the published article.

Simvastatin inhibits osteoclast differentiation by scavenging reactive oxygen species

Osteoclasts, together with osteoblasts, control the amount of bone tissue and regulate bone remodeling. Osteoclast differentiation is an important factor related to the pathogenesis of bone-loss related diseases. Reactive oxygen species (ROS) acts as a signal mediator in osteoclast differentiation. Simvastatin, which inhibits 3-hydroxy-3-methylglutaryl coenzyme A, is a hypolipidemic drug which is known to affect bone metabolism and suppresses osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand (RANKL). In this study, we analyzed whether simvastatin can inhibit RANKL-induced osteoclastogenesis through suppression of the subsequently formed ROS and investigated whether simvastatin can inhibit H2O2-induced signaling pathways in osteoclast differentiation. We found that simvastatin decreased expression of tartrate-resistant acid phosphatase (TRAP), a genetic marker of osteoclast differentiation, and inhibited intracellular ROS generation in RAW 264.7 cell lines. ROS generation activated NF-kappaB, protein kinases B (AKT), mitogen-activated protein kinases signaling pathways such as c-JUN N-terminal kinases, p38 MAP kinases as well as extracellular signal-regulated kinase. Simvastatin was found to suppress these H2O2-induced signaling pathways in osteoclastogenesis. Together, these results indicate that simvastatin acts as an osteoclastogenesis inhibitor through suppression of ROS-mediated signaling pathways. This indicates that simvastatin has potential usefulness for osteoporosis and pathological bone resorption.

Optimal combination of 3-component photoinitiation system to increase the degree of conversion of resin monomers / 대한치과보존학회지

OBJECTIVES: This study investigated the optimal combination of 3-component photoinitiation system, consisting of CQ, p-octyloxy-phenyl-phenyl iodonium hexafluoroantimonate (OPPI), and 2-dimethylaminoethyl methacrylate (DMAEMA) to increase the degree of conversion of resin monomers, and analyze the effect of the ratio of the photoinitiator to the co-initiator. MATERIALS AND METHODS: Each photoinitiators (CQ and OPP) and co-initiator (DMAEMA) were mixed in three levels with 0.2 wt.% (low concentration, L), 1.0 wt.% (medium concentration, M), and 2.0 wt.% (high concentration, H). A total of nine groups using the Taguchi method were tested according to the following proportion of components in the photoinitiator system: LLL, LMM, LHH, MLM, MMH, MHL, HLH, HML, HHM. Each monomer was polymerized using a quartz-tungsten-halogen curing unit (Demetron 400, USA) for 5, 20, 40, 60, 300 sec and the degree of conversion (DC) was determined at each exposure time using FTIR. RESULTS: Significant differences were found for DC values in groups. MMH group and HHM group exhibited greater initial DC than the others. No significant difference was found with the ratio of the photoinitiators (CQ, OPPI) to the co-initiator (DMAEMA). The concentrations of CQ didn't affect the DC values, but those of OPPI did strongly. CONCLUSIONS: MMH and HHM groups seem to be best ones to get increased DC. MMH group is indicated for bright, translucent color and HHM group is good for dark, opaque colored-resin.

Study on Determinants of Nursing Needs of Mothers with Hospitalized Children / 기본간호학회지

PURPOSE: The purpose of this study was to analyze the factors influencing nursing needs of mothers caring for children suffering from acute diseases and being treated in hospital. METHOD: The data were collected, using a nursing needs, social support, burden, and stress questionnaire. Data collection was done from July 10, to October 20, 2001 in the pediatric department of a general hospital. The data were analyzed by using descriptive statistics. RESULT: The results of this study are as follows. There were statistically significant differences in nursing needs according to differences in age, education level, marital status, religion, family income and relationship with spouse. The relationship between the nursing needs of the mother and anxiety (r=.758, p <.01), social support (r=-.659, p <.01), stress (r=.324, p <.01) were the most significant variables. The stepwise multiple regression analysis showed that anxiety, social support and stress define nursing needs at 57.5%, 4.3% and 1.1% respectively. CONCLUSION: Consideration needs to be given to anxiety, social support and stress when developing nursing intervention programs for mothers whose children are hospitalized with acute diseases.