RESUMEN
Background: Hepatitis C Virus (HCV) is one of thecauses resulting in chronic hepatitis, cirrhosis and primary hepatocarcinoma. In Viet Nam, HCV-infected people are on the increase. The co-existence of HCV and G (HGV) in Viet Nam and their accurate genotyping needs to be clarified. Objective: To detect and molecularly genotype HCV and HGV from 4 serum isolates. Materials: This study consisted of 4 anti-HCV positive [HCV(+)] serum isolates. Method: To extract genomic RNA, perform RT-PCR, 5\u2019UTR fragment of 295 nucleotides for HCV, genomic RNAs from HCV(+) (confirmed by RT-PCR) as samples for RT-PCR for HGV to obtain 260 bp 5\u2019UTR. All of them were cloned and sequenced for analysis. Results: HCV products and HCV + HGV products obtained from samples, respectively; suggested that the co-existence of HCV and HGV could occur in a patient. Base on Gen Back and analysis, we showed that three nucleotide strains of HCV (HCV-H1VN, HCV-H2VN, HCV-H3VN) belongs to genotype 1a of the group 1a/1b, commonly found in East and Southeast Asia. HGV of Viet Nam [HGV-(Han9)VN strain] was identified to belong to type 2; one of five HGV types existing in the world. Conclusions: Three HCV isolates were genotype 1a; one HGV isolate belonged to genotype 2. Detection and genotyping of co-existence of HCV and HGV contributed the development of a multiplex-PCR/RT-PCR for screening blood-transmitted viral hepatitis.
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GenotipoRESUMEN
Background: People were infected with Cysticercus by either eating Taenia solium eggs from the environment or from proglottids in intestines. These eggs hatched to larvae in muscular and cerebral tissue, which causes specific symptoms. \r\n', u'Objectives: To determine symptoms of human cysticercosis. \r\n', u'Subjects and methods: Clinical and para-clinical processes were conducted on 30 cysticercosis patients, of which 21 were male (70%) and 9 female (30%).\r\n', u'Results and conclusion: The main symptoms were headache in 29 patients (96.7%), subcutaneous cysts in 28 patients (93.3%), epilepsy in 14 patients (46.7%), positive ELISA (antigen of T.solium) with samples in 28 (93.3%) and positive ELISA with CSF samples (73.3%), living cysts in the brain were discovered in 25 patients (83.3%) by CT scanner and eosinophylia in 24 patients (80%). Cysticercus nodules were collected from 28 patients in this study; the species were identified by molecular method. A portion of 652 bp of mitochondrial-encoded cytochrome oxidase b (cob) and 217 amino-acid was amplified by Polymerase Chain Reaction (PCR) and sequenced. The nucleotide sequence was comparatively aligned with the known corresponding sequences of Taenia solium Chinese (TsoCN1). Molecular-based analysis revealed that the Cysticercus from the patients in this study was identified as Taenia solium. There is absolute nucleotide and amino-acid similarity between Taenia solium Chinese (hemogeny 99.1-99.8% of nucleotide and 100% of amino acid).\r\n', u'