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Journal of Southern Medical University ; (12): 12-15, 2008.
Artículo en Chino | WPRIM | ID: wpr-281493

RESUMEN

<p><b>OBJECTIVE</b>To observe the effects of amitrole on the transcription of thyroglobulin (tg), thyroid peroxidase (tpo), Na(+)/I- symporter (nis), Na(+)/I- symporter (nis), thyroid-stimulating hormone receptor (tshr), thyroid transcription factor 1 (ttf-1) and paired-domain protein-8 (pax-8) genes in FRTL-5 cells and investigate the mechanism of amitrole for intervening in thyroid hormone activity.</p><p><b>METHODS</b>FRTL-5 cells were treated with amitrole at 0.001, 0.01 and 0.1 mg/ml for 24 h, respectively, after which the cells were collected for extraction of the total RNA. RT-PCR was used to examine the effects of amitrole on the transcription of tg, tpo, nis, tshr, pax-8 and ttf-1 genes in FRTL-5 cells.</p><p><b>RESULTS</b>Amitrole significantly induced tg gene transcription at all the doses, but produced no obvious effects on tpo and nis gene transcription. At the concentration of 0.1 mg/ml, amitrole significantly reduced pax-8 and tshr gene transcription but increased ttf-1 gene transcription.</p><p><b>CONCLUSION</b>The effects of amitrole on thyroid hormone activity may be related with its actions on tg, ttf-1, tshr and pax-8 gene transcription.</p>


Asunto(s)
Animales , Ratas , Amitrol (Herbicida) , Toxicidad , Células Cultivadas , Inhibidores Enzimáticos , Toxicidad , Células Epiteliales , Biología Celular , Metabolismo , Proteínas Nucleares , Genética , Ratas Endogámicas F344 , Receptores de Tirotropina , Genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Tiroglobulina , Genética , Glándula Tiroides , Biología Celular , Factor Nuclear Tiroideo 1 , Factores de Transcripción , Genética , Transcripción Genética
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