RESUMEN
To investigate the feasibility of using recombinant adeno-associated virus type 1 vector as prophylactic vaccine against HPV16 infection, rAAV1-mod. HPV16L1, the recombinant AAV1 vector containing codon-modified HPV16 L1 gene, was constructed. C57BL/6 mice were immunized with purified rAAV1 vector through intramuscular and intranasal inoculation routes, and the titer of neutralizing antibody was determined by neutralization assay based on HPV16 pseudovirus. The result shows that the single dose of rAAV1-mod. HPV16L1 can induce specific neutralizing antibody in serum through both inoculation routes. Compared with intranasal group, intramuscular group can induce higher titer of neutralizing antibody. Eliciting strong and prolonged neutralizing antibody in serum, the rAAV1-mod. HPV16L1 is one of promising HPV16 prophylactic vaccine candidates.
Asunto(s)
Animales , Femenino , Ratones , Anticuerpos Antivirales , Sangre , Proteínas de la Cápside , Genética , Alergia e Inmunología , Dependovirus , Genética , Ratones Endogámicos C57BL , Proteínas Oncogénicas Virales , Genética , Alergia e Inmunología , Vacunas contra Papillomavirus , Alergia e Inmunología , Vacunación , Vacunas Sintéticas , Alergia e InmunologíaRESUMEN
<p><b>OBJECTIVE</b>To evaluate the immune potency of recombinant adenovirus combined with rAAV1 vector expressing HPV16L1 protein in mice.</p><p><b>METHODS</b>The rAdV and rAAV1 vector containing codon-modified HPV16L1 gene was constructed using Admax and AAVmax packaging system respectively. C57 BL/6 mice were immunized with purified rAdV and rAAV1 vector through intramuscular and intranasal inoculation routes, and the titer of neutralizing antibody was determined by neutralization assay based HPV16 pseudovirus.</p><p><b>RESULTS</b>Intramuscular immunization by rAAV1-mod. HPV16L1 or combined with rAd-mod. HPV16L1 can induce higher titer of neutralizing antibody in serum than that of other groups. The titer of neutralizing antibody of intranasal groups is significantly lower than that of intramuscular group, although the prime-boost strategy using in intranasal group was effective to enhance the specific humoral immunity.</p><p><b>CONCLUSION</b>The rAAV1-mod. HPV16L1 combined with rAd-mod. HPV16L1 can induce higher titer of neutralizing antibody in serum through intramuscular route than that of other groups at the 16th week after the first immunization.</p>
Asunto(s)
Animales , Ratones , Adenoviridae , Genética , Alergia e Inmunología , Infecciones por Adenoviridae , Alergia e Inmunología , Anticuerpos Antivirales , Alergia e Inmunología , Dependovirus , Genética , Alergia e Inmunología , Fenómenos del Sistema Inmunológico , Inmunización , Ratones Endogámicos C57BL , Proteínas de Fusión Oncogénica , Alergia e Inmunología , Proteínas Oncogénicas Virales , Alergia e Inmunología , Proteínas Recombinantes , Genética , Alergia e InmunologíaRESUMEN
<p><b>OBJECTIVE</b>To study the effect of anti-HIV III B virus with extractions from Juglans regia, so as to searching for the new and efficacious leading compound of AIDS therapy.</p><p><b>METHOD</b>Phytochemical and chromatographical techniques were used to isolate compounds from J. regia; MT4 cells and HIV III B virus were used to study the effect of anti-HIV activity in vitro. BIACORE 3000 molecule coupled equipment were used for the target research also.</p><p><b>RESULT</b>Two extractions (B&E) were isolated from J. regia which possess the effect of anti-HIV activity. Targets study found that extraction B could affected on HIV-1 gp-41 fusing protein and extraction E could affected on HIV-1 integrase respectively.</p><p><b>CONCLUSION</b>J. regia is a traditional Chinese medicine with active anti-HIV activity and worth to be studied further.</p>
Asunto(s)
Humanos , Línea Celular , Medicamentos Herbarios Chinos , Química , Farmacología , VIH-1 , Juglans , Química , Extractos Vegetales , Química , FarmacologíaRESUMEN
<p><b>OBJECTIVE</b>To evaluate the mastication function of the patient whose maxilla was reconstructed with individual titanium mesh and Chinese flap or combined with fibular flap. The superiority of this method on maxillary functional reconstruction was testified.</p><p><b>METHODS</b>Since March of 2001, 10 cases of maxillary defect were reconstructed with individual titanium mesh and Chinese flap or combined with fibular flap, and routine removal partial dentures were fixed after 3 - 6 months postoperatively. Assessment of occlusal force was proceeded by T-Scan II system (Tekscan company, USA).</p><p><b>RESULTS</b>The occlusal force analysis results indicated the asymmetry index of bite force and asymmetry index of occlusal contact area differed significantly between preoperation and postopertion (P < 0.05). The recovery rate of total occlusal force was between 27.05%-74.06%, and the average was 50.15%.</p><p><b>CONCLUSION</b>This new three-dimensional maxillary functional reconstruction method had a satisfactory recovery in both of contour and function recently, especially restored the mastication function effectively.</p>