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Chinese Journal of Medical Genetics ; (6): 709-711, 2005.
Artículo en Chino | WPRIM | ID: wpr-263866

RESUMEN

<p><b>OBJECTIVE</b>To investigate the distributions of six short-tandem repeat (STR) loci, namely D7S820, D13S317, D16S539, HUMCSF1PO, HUMTPOX and HUMTH01, in Miao minority group at Rongshui county in Guangxi province and construct the relevant genetic database.</p><p><b>METHODS</b>Sodium-citrated blood specimens were collected from 208 healthy unrelated Miao individuals in Rongshui county. The DNAs from the specimens were extracted with phenol-chloroform method; AmplFSTR Identifier PCR Amplification Kit was used to amplify the extracted DNAs, and 3100 Genetic Analyzer was used to analyze and screen the amplified products.</p><p><b>RESULTS</b>In this study, 7, 8, 6, 7, 5, 7 alleles were observed at the 6 STR loci respectively. The expected distribution of genotype accorded with Hardy-Weinbery equilibrium. The total discrimination power, cumulative paternity exclusion power and total polymorphism information were 0.999995, 0.9959 and 0.9987 respectively.</p><p><b>CONCLUSION</b>The results demonstrate that these 6 STR loci are of high polymorphism and hereditary stability and are in accord with Mendel's law. The data obtained are valuable in population genetics research, forensic application, and individual identifications.</p>


Asunto(s)
Adolescente , Niño , Humanos , China , Etnicidad , Genética , Frecuencia de los Genes , Desequilibrio de Ligamiento , Repeticiones de Microsatélite , Genética , Reacción en Cadena de la Polimerasa , Polimorfismo Genético
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