A porcine gene, PBK, differentially expressed in the longissimus muscle from Meishan and Large White pig

An investigation of differences in gene expression in the longissimus muscle of Meishan and Large White pigs was undertaken, using the mRNA display technique. A fragment of one differentially expressed gene was isolated and sequenced, whereupon the complete cDNA sequence was then obtained by using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of the gene is not related to any known porcine gene. Sequence analysis revealed that the open reading frame of this gene encodes a protein with 322 amino acids, thus displaying high sequence identity with the PDZ binding kinase (PBK) of eleven other animal species - dog, horse, cattle, human, chimpanzee, crab-eating macaque, rhesus monkey, rat, mouse, gray short-tailed opossum and platypus, so it can be defined as the porcine PBK gene. This gene was finally assigned GeneID: 100141310. Phylogenetic tree analysis revealed that the swine PBK gene has a closer genetic relationship with the PBK gene of platypus. Gene expression analysis of eight tissues of a Meishan x Large White cross showed that the porcine PBK gene is differentially expressed in various tissues. Our experiment established the primary foundation for further research on this gene.

Determination of Penicillin G in Human Plasma by RP-HPLC / 中国药房

OBJECTIVE:To establish the method to determine Penicillin G in human plasma by RP-HPLC.METHODS:C 18 was used as the chromatographic column.The mobile phase consisted of 0.05 mol/L phosphate buffer solution-acetonitrile(75∶25)with Penicillin V as the internal standard.The plasma sample introduction was carried out after purified and derivatized by C 18 SPE.The determination was carried out when the wavelength was at 325nm.The quantitation was done by internal standard method.RESULTS:Linear concentration range for penicillin was 0.05～20?g/L;the concentration limit was 0.05?g/L;the intraday RSD was less than 2.5% and the method recovery rate was within the range of 95%～104%.CON?CLUSION:This method was simple,rapid,sensitive and accurate,which can be used to determine the blood concentration of penicillin.