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1.
Chinese Journal of Clinical Laboratory Science ; (12): 392-395, 2018.
Artículo en Chino | WPRIM | ID: wpr-694852

RESUMEN

Objective To investigate the role of miR-374-5p in regulating mesenchymal stem cells ( MSCs) derived from gastric tissues to promote the proliferation and migration of gastric cancer cells in vitro. Methods The gastric cancer-mesenchymal stem cells ( GC-MSC) and the matched paracancerous tissue of gastric cancer derived normal mesenchymal stem cells ( GCN-MSC) were isolated and cultured in vitro. Gastric cancer cells were treated with the culture supernatants of GCN-MSC and GC-MSC respectively in vitro. The proliferation ability of gastric cancer cells was detected by plate clone test and the migration ability was detected by scratch test. MiR-374-5p mimics were used to over-express miR-374-5p in GC-MSC and GCN-MSC and MiR-374-5p inhibitor was used to inhibit its expression level. The culture supernatant of MSCs after transfection was collected and gastric cancer cells were treated in vitro. The changes of proliferation and migration ability of gastric cancer cells were observed. Results The number of single cell colonies formed in GC-MSC culture supernatant group (112±7) was more than that of GCN-MSC group (48±6) and the distance between the cells (6.5±0.5 cm) was less than that of GCN-MSC group (15±1) cm, by which the proliferation and migration of gastric cancer cells were significantly promoted (t=12.02, P<0.01 and t=13.17, P<0.01,respectively). Following miR-374-5p overexpressing in the culture supernatant of GCN-MSC,the number of single cell colonies increased (115±12.1) and space between cells reduced (7.83±1.08 cm), by which the proliferation ( t=9.31, P<0.01) and migration ( t=4.88, P<0.01) of gastric cancer were also promoted. The miR-374-5p inhibitor reversed the GC-MSC-induced ability of proliferation and migration of gastric cancer ( t=5.47,P<0.01 and t=4.95,P<0.01) . In the inhibitor group the number of single cell colonies decreased (60±10.2) and the space between cells increased (12.17±1.47cm) following treating by the culture supernatant. Conclusion MiR-374-5p should be an important molecule that regulates MSC in tumoral microenvironment to promote the proliferation and migration of gastric cancer and may become a prospective target for diagnosis and treatment of gastric cancer.

2.
Chinese Journal of Applied Clinical Pediatrics ; (24): 499-502, 2016.
Artículo en Chino | WPRIM | ID: wpr-489749

RESUMEN

Objective To study the role of early intravenous nutrition given aggressively combined with early minimal feeding on very low birth weight infants (VLBWI),and to evaluate the clinical value of intestinal barrier protein and MicroRNA.Methods All of 62 cases of VLBWI admitted in NICU,the Maternal and Child Health Hospital of Nantong Affiliated to Nantong University from January 2006 to June 2014 were recruited.Sixty-two VLBWI were randomly divided into group A and group B.Thirty infants in group A were exposed to conventional intravenous nutrition.Thirty-two infants in group B were treated with early intravenous nutrition aggressively combined with early minimal feeding.The time of birth weight recovery,days with intravenous nutrition,hospital stay and complications were recorded.The liver and kidney functions,electrolytes,blood gas analysis were monitored.Enzyme-linked immunosorbent method was used to detect intestinal fatty acid binding protein (Ⅰ-FABP),an intestinal barrier protein in plasma.Infection related MicroRNA155 was detected with fluorescent quantitative polymerase chain reaction (RT-PCR).Results Group B was superior to group A in weight loss after birth [(13.70 ± 3.10) % vs (5.46 ± 2.64) %,P < 0.05],shorter recovery time of body weight [(12.20 ± 3.38) d vs (6.82 ± 3.20) d,P < 0.05],fewer days with intravenous nutrition [(29.62 ± 4.16) d vs (20.80 ± 3.20) d,P < 0.05] and shorter hospital stays [(44.60 ± 6.32) d vs (28.91 ± 4.36) d,P < 0.05].Compared with group A,the infants in group B had less complications,including hyperbilirubinemia (31.2% vs 56.7%),extrauterine growth retardation (34.3% vs 73.3%),cholestasis (6.2% vs 23.3%),feeding intolerance (15.6% vs 53.3%) and necrotizing enterocolitis (0 vs 16.7%) (all P < 0.05).Although Ⅰ-FABP had a higher plasma concentration in group A than that of group B [(9.083 ± 1.059) μg/L vs (7.563 ± 0.739) μg/L],the difference was not significant (t =1.190,P =0.076 4).However,the plasma levels of Ⅰ-FABP in infants with necrotizing enterocolitis were significantly higher than those of group B [(19.500 ± 3.510) μg/L vs (7.563 ±0.739) μg/L,t =5.231,P =0.035 0].The expression of MicroRNA155 in group A was markedly higher than that of group B (2-△△ct were 0.81 ± 0.12 and 0.24 ± 0.08,respectively,P < 0.05).Conclusions Giving aggressive intravenous nutrition early combined with early minimal feeding was safety and effective to VLBWI,which was of benefit to their growth and development,reducing complications and shorting hospital stays.The detection of intestinal barrier protein Ⅰ-FABP and MicroRNA155 is useful for monitoring feeding complications of VLBWI.

3.
Chinese Journal of Urology ; (12): 48-51, 2016.
Artículo en Chino | WPRIM | ID: wpr-488684

RESUMEN

Objective To investigate the clinical features, diagnosis, treatment and prognosis of primary testicular diffuse large B-cell lymphoma.Methods 8 patients with primary testicular diffuse large B-cell lymphoma were reviewed from April, 2012 to April, 2015.The mean age was 58 years old, ranging 43-68 years old.Color Doppler echocardiography examination showed that there were round or oval regular tumors in the scrotum, which the diameter ranged from 3.5 to 8.0 cm, mean 5.5 cm.There was no abnormal changes among abdomen and pelvic cavity in CT scan and tumor markers examination.The radical orchiectomy were performed in all patients.After opening the tunica vaginalis, a hard texture tumor could be found, which has the vague border line with normal tissue.All patients were diagnosed according to the combination of morpbologic and immunohistochemical examination after operating.Results All patients accepted operation successfully.The mean operation time was 34 minutes, ranging 25-40 minutes.8 cases were diagnosed as primary testicular diffuse large B-cell lymphoma after operating.Immunohistochemical analysis showed that CD20, BCL-6 were positive.CD3, CD10, CK were negative.All patients received adjuvant chemotherapy with RCHOP(cytoxan 750 mg/m2, adriamycin 50mg/m2, leurocnstine 1.4 mg/m2, prednisone 60 mg/m2 ,rituximab 375 mg/m2) regimen over 6 cycles, which was conducted once every three weeks, one week post-operatively.The follow up duration ranged from 6 to 36 months, mean 17 months.All patients survived at the end of this study with no sign of recurrence and metastasis.Conclusions The patients with primary testicular diffuse large B-cell lymphoma are rare.The radical orchiectomy is recommended.And RCHOP chemotherapy should be considered one week post-operatively.The short term outcome of the treatment is satisfactory.But the long term outcome should be further studied.

4.
Chinese Journal of Urology ; (12): 591-594, 2010.
Artículo en Chino | WPRIM | ID: wpr-387391

RESUMEN

Objective To investigate the effect of reptroperitoneal laparoscopic operation on the parameters of platelet, D-dimer and thrombomodulin(TM). Methods Forty cases were divided into two groups according to the operative way, retroperitoneal laparoscopic operation (n= 20) and open operation (n=20). Blood samples were taken preoperatively and at the end of the surgery. The following parameters were measured and compared within each group and between groups: platelet count (PLT), mean platelet volume (MPV), platelet distributionwidth(PDW), D-dimer, TM. ResultsThere were no significant differences for the PLT, PDW, MPV, TM and D-dimer between before and after operation in each group. There was no difference between 2 groups either for all these indicators.No patients from either group suffered thrombosis or abnormal bleeding as a pastoperative complication. Conclusion Compared with the conventional operation, retroperitoneal laparoscopic operation dioesd not induce more change on parameters of platelet, D-dimer and TM.

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