Comparative Analysis of Volatile Oil Constituents of Glehnia littoralis from Different Producing Areas / 中国药房

OBJECTIVE： To compare the difference in volatile oil constituents of Glehniae littoralis from 3 producing areas as Shandong Laiyang， Hebei Anguo， Inner Mongolian Chifeng. METHODS： The method of steam distillation was used to extract the volatile oil of G. littoralis from different areas and calculate the extraction rate. The constituents of volatile oil were analyzed by using GC-MS. The data was corrected by Xcalibur chemical workstation. The constituents were searched by NIST 11.0 mass spectrometry database （matching degree ＞800）， and the relative mass fraction of each chemical constituent was obtained by peak area normalization. RESULTS： The extraction rate of volatile oil in G. littoralis from Laiyang was 0.013％， which was far lower than G. littoralis from Anguo （0.099％） and G. littoralis from Chifeng （0.105％）. There were 15， 18 and 27 constituents identified in volatile oil of G. littoralis from 3 producing areas； the relative mass fractions were 89.29％， 96.76％， 94.53％. Falcarinol was a common compound with the highest relative mass fraction of the volatile oil of G. littoralis from different producing areas； the relative mass fractions were 69.79％， 90.89％ and 71.04％， respectively. Fatty acids were rich in the sample from Laiyang， while C15H24 sesquiterpenoids were rich in the other samples from Anguo and Chifeng. CONCLUSIONS： Volatile oil of G. littoralis could be used as potential chemical markers to distinguish different producing areas due to their significant differences in chemical components.

Predictive value of 18F-FDG PET-CT for lymph node metastasis of peripheral lungadenocarcinoma / 医学研究生学报

Objective Classification of non-small cell lung lymph (NSCLC) node (N) is one of the key factors influencing treatment, however, the cilinical noninvasive and invasive approaches to N classification have their limitations.This study aimed to investigate the risk factors of lymph node metastasis of peripheral lungadenocarcinoma by using CT and PET / CT scans.Methods Retrospective analysis had been done on a total of 248 patients who underwent surgical resection from February 2010 to November 2015 in our hospital.All of them underwent chest CT and 80 patients underwent PET/CT examination.Univariate analysis was applied in the relation of lymph node metastasis to gender, age, smoking situation, CEA, SUV, cancer size, pathological variants, and the degree of differentiation.Multivariable logistic regression analysiss were performed in the prediction of risk factors for lymph node metastasis.ResultsSeventy-four patients (29.8%) had regional lymph node metastases.Univariate analysis showed that lymph node metastasis was related to the serum CEA level, degree of differentiation, SUVmax, tumor size, lobulation/spiculation, pleural retraction, mediastinal or hilar lymphadenopathy (P<0.05).In the multivariable analysis of risk factors, including serum CEA, SUVmax and CT features, for predicting lymph node metastasis, the most important and significantly independent risk factors identified were SUVmax, CEA level, mediastinal or hilar lymphadenopathy, cavitation/bubble-likelucency and pleural retraction (P<0.05).Conclusion The lymph node metastasis is associated with SUVmax of primary tumor, serum CEA level, mediastinal or hilar lymphadenopathy, cavitation/bubble-likelucency and pleural retraction.The combination of radiographic features and serum CEA can help to predict more accurately the risk of lymph node metastasis in patients with peripheral lung adenocarcinoma.

A Comparative Assessment of Voice after Laser Surgery Versus Radiotherapy of T1a Glottic Carcinoma:A Meta-Analysis / 听力学及言语疾病杂志

Objective To carry out a Meta-analysis on the published data to evaluate voice after laser surgery versus radiotherapy of T1a glottic carcinoma.Methods The major medical-related databases were used in this study including PubMed, Web of Science,Embase, Cochrane library, CNKI and Wanfang Date.The articles by two co-authors at the same time were screened to read the relevant literatures, extraction of data and research related to the purpose from the selected literature.Results According the necessary conditions,fourteen literatures was included finally, and statistical analysis results showed that owing to the meta-analysis of the maximum time of speech (MPT) [test for overall effect:Z=2.65,MD=-2.26,95%CI(-3.94,-0.59),P=0.008] and the fundamental frequency (F0) [test for overall effect:Z=7.49,MD=14.41,95%CI(10.14,18.19),P<0.000 01], radia-tion therapy was superior to laser surgery,but the Meta-analysis of voice handicap index (VHI) [test for overalleffect:Z=1.04,MD=5.86,95%CI(-5.22,16.94),P=0.30], jitter[test for overall effect:Z=1.43,MD=0.75,95%CI(-0.28,1.79),P=0.15], shimmer[test for overall effect:Z=1.26,MD=1.07,95%CI(-0.60,2.75,P=0.21] and Airflow rate(AFR) [test for overall effect:Z=0.42,MD=21.46,95%CI(-78.79,121.72),P=0.67] don''t show statistically significant difference between the two groups.Conclusion The radiation therapy is slightly superior to the laser surgery in protecting voice in the treatment of T1a glottic carcinoma.

Nasal primitive neuroectodermal tumor: a case report / 临床耳鼻咽喉头颈外科杂志

Primitive neuroectodermal tumor (PNET) is a small round cell malignancies, normally confined to the chest wall and soft tissues of children and young adults, and reports of this tumor existing in nasal cavity are scarce. We herein present a case of nasal PNET. A 35-year-old male patient presented with a chief complaint of stuffy nose was diagnosed with a primary nasal pPNET by the clinical, microscopic, immunohistochemical features. Since these tumors are highly aggressive and patient prognosis is typically very poor, early diagnosis, immediate surgical resection and re-resection if possible, early radiotherapy and chemotherapy and close follow-up are required. We also review literatures on pPNET.

Analysis of surgical methods and clinical analysis of its long-term effect of laryngeal carcinoma / 中国癌症杂志

Background and purpose:Surgical operation is the main method in the treatment of laryngeal carcinoma, but different patients have different impacts on the survival time and the quality of life with different type of operation. This study was to analyze the methods of surgical treatment and its long-term effect of laryngeal carcinoma, to increase survival rates, laryngeal function reservation and reconstruction and improve life quality.Methods:A total mumber of 424 patients of laryngeal carcinoma treated with surgical treatment during between Jan. 2002 and Dec. 2012 were researched by clinical follow-up and data analysis. Surgical method: CO2 laser-assisted laryngeal microsurgery for laryngeal tumors for 50 cases, frontal partial laryngectomy or modiifed thyroid cartilage window partial laryngectomy without tracheostomy for 42 cases, vertical frontolateral partial laryngectomy for 119 cases, horizontal partial laryngectomy and extended subtotal laryngectomy for 22cases, anastomosis of pharynx and trachea for 4 cases, supra cricoid partial laryngectomy for 129 cases (CHEP of them for 103 cases, CHP for 26 cases), total laryngectomy for 58 cases, cervical lymph node dissection at the same term for 121 cases.Results:Partial laryngectomy without tracheostomy for 92 cases (21.7%), total laryngectomy for 58 cases (13.7%); decannulation rate was 86.5%, vertical frontolateral partial laryngectomy of them for 93.2%, horizontal partial laryngectomy of them for 90.9%,supra cricoid partial laryngeal of them for 82.2%; laryngeal function reservation rate for 86.3%; all patients did outpatient review and telephone follow-up, 9 cases loss to follow-up, a total of tumor recurrence, cervical lymph node metastasis and distant metastasis were 41 cases, mostly occurred in 1 year after surgery; death for 57 cases, relapse of them for 8 cases, cervical metastasis for 13 cases, pulmonary metastasis for 5 cases, hepatic metastases for 2 cases, brain metastases for 1 case, esophagus metastases for 1 case, pulmonary infection for 6 cases, acute renal failure for 2 cases, unknown reason for 19 cases, according to Kaplan-Meier to count survival rate, 3-year and 5-year survival rate were 90.7% and 84.1%, relapse and metastasis were the main causes of death.Conclusion:Surgical treatment is the main therapy mode of laryngeal carcinoma. We choose individualized surgical methods for patients according to tumor staging, invasion site, age, occupation and education background of patient, health condition and so on. On the premise that tumor is completely cut off, we always advocate function surgery and minimally invasive surgery, and adopt comprehensive treatment at the same time, in order to increase survival rates, lesson suffering and improve life quality as far as possible.

Gastroesophageal reflux and otitis media with effusion / 临床耳鼻咽喉头颈外科杂志

Otitis media with effusion (OME), a kind of inflammatory disease of the middle ear, is characterized by the retention of middle ear effusions and hearing loss. There are many factors involved in the development of OME including bacterial infection, formation of bacterial biofilms, eustachian tube dysfunction, adenoid hypertrophy, immune system disorders. Recently, some studies showed that gastroesophageal reflux has correlation with OME.

A systematic review of adenoidectomy in the treatment of otitis media with effusion in children / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#Systematic reviews and Meta-analysis were conducted to assess the efficacy and security of adenoidectomy for the treatment of otitis media with effusion in children.@*METHOD@#Based on the principles and methods of Cochrane systematic reviews, literature was searched in PubMed, Medline, Elisevier, Ovid, CBM, CNKI, VIP and Wanfang datebases. Randomized controlled trials about treatment of otitis media with effusion in children using adenoidectomy were included. Meta-analysis was performed for the result of homogeneous studies using RevMan 5.2 software.@*RESULT@#Adenoidectomy (combined with myringotomy or puncture) was superior to non-surgical (combined with myringotomy or puncture) treatment in reducing the incidence of acute otitis media and removing the middle ear effusion. Adenoidectomy combined with tympanostomy tube was superior to tympanostomy tube alone in the removal of the middle ear effusion and improvement of hearing level. Three trials described some postoperative complications including haemorrhage, incipient malignant hyperthermia, postoperative pneumonia and velopharyngeal insufficiency.@*CONCLUSION@#Our research shows a benefit of adenoidectomy in the removal of middle ear effusion in children with OME. Adenoidectomy combined with tympanostomy tube was superior to tympanostomy tube alone in improving hearing level. At present, there is no evidence of serious postoperative complications after adenoidectomy.

Observation of postoperative recovery time with different surgical procedures for treatment of vocal polyps / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To compare postoperative voice recovery time of vocal polyps treated with different power levels of CO2 laser and conventional laryngeal microsurgery on vocal polyps.@*METHOD@#Two hundred patients with vocal polyps were randomly divided into four groups, which were respectively treated with conventional laryngeal microsurgery and different power levels of CO2 laser (2 W, 4 W and 6 W) under laryngoscope. The postoperative voice recovery time were analyzed.@*RESULT@#All vocal polyps were resected at one time without any serious complications like dyspnea or bleeding. The postoperative voice recovery time were 7.2 days for the group of patients received conventional laryngeal microsurgery and 7.6 days for the group of patients treated with 2 W CO2 laser, which were hoch significantly different from that in the group of patients treated with 6 W CO2 laser (P < 0.05).@*CONCLUSION@#The postoperative recovery time of patients treated with conventional laryngeal microsurgery and small power of CO2 laser is shorter than those operated with the other larger power of CO2 laser.

Clinical utility of tuberculosis protein chip in diagnosis of tuberculosis / 中国感染与化疗杂志

Objective To explore the potential value of tuberculosis protein chip for clinical diagnosis of tuberculosis.Methods The antibody level of tuberculosis protein ESAT-6,CFP10,16 KD,38 KD and LAM was determined in 4 093 patients,inclu-ding 441 tuberculosis and 3 652 non-tuberculosis cases by protein chip.Results The tuberculosis antibody was positive in 297 of the 441 tuberculosis cases and 647 of the 3 652 non-tuberculosis cases.Tuberculosis protein chip provided a sensitivity of 67.35% and specificity of 82.28% in the diagnosis of tuberculosis.Conclusions Tuberculosis protein chip test is a quick,easy and effective method for identifying potential tuberculosis patients with good specificity.

Platelet-rich plasma versus corticoid in the treatment of chronic lateral elbow epicondylitis / 中华全科医师杂志

A total of 52 patients with chronic lateral epicondylitis were randomly assigned into platelet-rich plasma (PRP) (n =28) or corticosteroid (n =24) group.The visual analog pain scale (VAS) and Mayo elbow score had no significant difference between two groups within 1 week post-treatment.However,VAS and Mayo elbow score improved more significantly in PRP group versus corticosteroid group at Month 1 and 6 post-treatment (P ＜ 0.05).The PRP trigger point injection treatment of elbow lateral epicondylitis achieved clinical outcomes and it was superior to glucocorticoid treatment.

Surgical methods and clinical analysis of early-stage glottic carcinoma involved anterior commissure / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To explore the surgical methods for management of early-stage glottic carcinoma involved anterior commissure.@*METHOD@#Fifty-eight cases of early-stage glottic carcinoma (T1, n = 23; T2, n = 35) treated with vertical frontolateral partial laryngectomy and frontal partial laryngectomy or modified window partial laryngectomy without tracheostomy were retrospectively reviewed.@*RESULT@#All patients can swallow smoothly and phonate clearly after operation. And the extubation rate with vertical fronto vertical partial laryngectomy is 97.2%. Tracheostomy was performed in one case after operation because of dyspnea. The most common postoperative complication was subcutaneous emphysema which developed postoperatively in 6 patients, but subsided after discharge. Follow-up was conducted for 8 months to 7 years, recurrence was observed in 3 cases. The 3 and 5 year survival rates were 97.5% and 91.8% respectively.@*CONCLUSION@#To choose the optimal surgical method for patients with early-stage glottic carcinoma not only can reduce the risk of recurrence but also can save the laryngeal function and thus improves the quality of life.

A case of fibrous dysplasia of temporal bone / 临床耳鼻咽喉头颈外科杂志

Osteofibrous dysplasia is a kind of innocent bone tumor derived from fibrous tissue. Its pathogens has not been identified, it rarely affects the temporal bone, its clinical manifestations mainly is hearing loss. Imageological examination, for example, CT and MRI, is valuable for the diagnosis of this disease,the histopathological evidence is absolutely necessary to make definite diagnosis. Surgery is an effective method to treat the fibrous dysplasia of temporal bone.

The Development and Application of a New Blood Component——the Cold Supernatant / 医学研究杂志

Objective To investigate the value of the cold supernatant in the clinical practice. Methods 25 cases of TTP got a direct infusion of cold supernatant or plasma exchange using cold supernatant as fluid replacement and with medications at the same time. Then we observed the clinical treatment effect of all the cases. Results The clinical remission rate of 25 cases of TTP was 80%. Conclusion The cold supernatant could be used as a new blood component in clinical application,especially for the treatment of thrombotic microangiopathy (TMA). It not only can save valuable blood resources,but also can reduce the economic costs of patients.

Functional outcomes after supracricoid partial laryngectomy / 中国耳鼻咽喉头颈外科

OBJECTIVE To evaluate the functional outcomes in patients undergoing supracricoid partial laryngectomy(SCPL).METHODS Twenty-one cases underwent supracricoid partial laryngectomy with functional reconstruction.In these cases.14 were glottic cancers(cricohyoidoepiglottopexy,CHEP),7 were supraglottic cancers(Cricohyokdopexy,CHP). RESULTS The mean time of decanulation was36.4 days,decannulation rate was 90.5%(19/21).All the patients recovered the function of speech and swallowing,but had variously hoarse.CONCLUSION The SCPL technique is effect in laryngeal functional reservation of laryngeal neoplasms.

Study on the isolation of marine fungi , screening of their antitumor activity and fermentation conditions / 中国海洋药物

Objective To isolate fungi from sea sediment and sea water samples collected from the neritic environment in Qingdao, screen their antitumor activity and study on their fermentation condition. Methods The antitumor activity was assayed by flow cytometry in mouse tsFT210 cells. Results The medium added penicillin and streptomycin possesses good selectivity. 207 strains of fungi were isolated and among them, 19 strains showed antitumor activity, and one fungus(Z 83200)pocesses strong cell apoptosis inducing activity.