Infiltration and effect of CD68+ and CD163+ macrophages in oral squamous cell carcinoma / 重庆医学

Objective To investigate the correlation between the number of CD68+ and CD163+ macrophages infiltration and the clinical characteristics of oral squamous cell carcinoma (OSCC),and to investigate the role and significance of CD163+ and CD68+ macrophages in OSCC.Methods Forty-two cases of OSCC resected by operation and diagnosed by postoperative pathology with intact clinical data in our hospitals during 2013-2016 were selected.Other 12 cases of oral mamaxillofacial normal tissue served as controls.The number of CD68+ and CD163+ macrophages infiltration in OSCC was detected by immunohistochemistry.Its correlation with clinical characteristics was analyzed.Results The number of CD68+ and CD163+ macrophages infiltration in OSCC was significantly higher than that in the control group (P<0.05);the infiltration quantity of CD68+ and CD163+ macrophages in poor differentiation and moderate differentiation was significantly higher than that in high differentiation(P<0.05).The number of CD68+ macrophages in lymph node metastasis was significantly lower than that without lymph node metastasis (P<0.05).The number of CD163+ macrophages in the lymph node metastasis was significantly higher than that of CD68+ (P<0.05).The number of CD68+ and CD163+ macrophages had positive correlation in OSCC (r=0.48,P=0.00).Conclusion Tumor-associated macrophages (TAMs) and M2 macrophages may play an important role in OSCC,TAMs may inhibits lymph node metastasis in OSCC,while M2 marcophages may promote the lymphatic matastasis of OSCC.

Comparison of conventional and new mesh hydroxyapatite in repair of cleft lip and palate / 中国组织工程研究

BACKGROUND:Despite regular mesh hydroxyapatite has certain biocompatibility and bone conductivity, it cannot be absorbed by human body after transplantation, and wil affect the growth and eruption of maxila and fangs when using for filing the injured alveolar cleft. OBJECTIVE: To compare the effect of conventional and new mesh hydroxyapatite composites in repair of alveolar cleft defects. METHODS: Totaly 46 patients with cleft lip and palate were randomly and evenly divided into test and control groups, and al were arranged according to cleft lip and palate sequence treatment. After nasal soft tissue defect repair, new mesh hydroxyapatite composite bone material (hydroxyapatite and bone morphogenetic protein compound). Conventional mesh hydroxyapatite material was adopted in the test and control groups, respectively. Within 6 months after the repair, the vertical distance from the newly formed bone tissue plane to the top of alveolar ridge was observed. The clinical repair effect was comprehensively evaluated from the aspects of complications, alveolar cleft shape and size, calus formation and material absorption. RESULTS AND CONCLUSION: At 2, 4, 8, 12 and 24 weeks, the vertical distance from the newly formed bone tissue plane to the top of alveolar ridge in the test group was higher than that in the control group (P < 0.05). The clinical repairing significant efficiency and total effective rate were al higher than those in the control group (P< 0.05). These results show that new mesh hydroxyapatite composite bone materials in the repair of alveolar cleft defects have good bone osteoinductivity and biocompatibility, rare complications and rapid calus formation.

Expression of Twist and relation with epithelial-mesenchymal transition in oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The objective of this paper was to study the expression of related protein and Twist transcription factor of epithelial-mesenchymal transition in oral squamous cell carcinoma (OSCC) tissue and the correlations of OSCC and oral squamous cell carcino-metastasis. The paper also investigated the clinical significance of expression on OSCC.</p><p><b>METHODS</b>The labels of epithelium materialization (E-cadherin and cytokeratin), stromal labels (N-cadherin), transcription factor Twist protein, and mRNA expression in 30 OSCC tissues were investigated via immunohistochemistry and in situ hybridization. The paper also conducted contrast analysis with clinicopathology.</p><p><b>RESULTS</b>Immunization result showed that the expressions of Twist and N-cadherin in the OSCC group were more significant than those of the normal group (P<0.05). The expressions of E-cadherin and keratin in OSCC were significantly lower than those of the normal group (P<0.05). In the moderate- and low-differentiated group of OSCC, the expressions of Twist and N-cadherin were higher than those of the high-differentiated group (P<0.05). The expressions of E-cadherin and keratin were lower than those in the high-differentiated group (P<0.05). In the lymphatic metastasis group, the expressions of Twist and N-cadherin were higher than those of no-lymphatic metastasis group (P<0.05). The expressions of E-cadherin and keratin were lower than those of the no-lymphatic metastasis group (P< 0.05). Results of in situ hybridization showed that the expression of Twist mRNA in the moderate- and low-differentiated groups of OSCC, T3, and T4 groups as well as that of the lymphatic metastasis group were higher than those of the high-differentiated, T1 and T2 groups, and no-separate lymphatic metastasis group, and the differences were statistically significant (P<0.05).</p><p><b>CONCLUSION</b>Epithelium materialization exists in OSCC tissue. Twist can enhance the invasiveness of tumor cell and promote the infiltration and metastasis of OSCC. The combined detection of Twist, E-cadherin, and N-cadherin expressions can effectively predict and estimate OSCC metastasis.</p>

An experimental study on cytotoxicity of monocytes/macrophages against Tca8113 cells in hypoxic microenvironment / 实用口腔医学杂志

Objective; To investigate the cytotoxicity of monocytes/macrophages against Tca8113 cells in hypoxic microenvironment in vitro. Methods; Peripheral blood mononuclear cells were extracted from healthy person

The expression and function of heparanase in the occurrence and evolvement of oral squamous carcinoma / 实用口腔医学杂志

Objective:To investigate the expression of Hpa and its correlation with the microvessel density(MVD) in oral squamous-cell carcinoma(OSCC), thereby learning the function of Hpa in the tumor angiogenesis,invasion and lymph node metastasis.Methods:The expression of Hpa was studied in 48 patients with OSCCs, 15 patients with leukoplakia and 10 normal person with healthy oral mucosa by conventional immunohistochemistry and in situ hybridization. MVD was assessed with anti-CD31 antibody. Results: Positive expression ratios of Hpa and Hpa mRNA in leukoplakia were significantly higher than those in normal oral mucosa, while were significantly lower than in OSCC(P

The expression of angiopoietin-2 and its correlation with tumor angiogenesis in oral squamous cell cacinoma / 实用口腔医学杂志

Objective:To study the role of angiopoietin-2(Ang-2) in angiogenesis in oral squamous cell cacinoma(OSCC). Methods:Expression of angiopoietin-2 and CD_34 in 41 cases of OSCC(13 cases with lymph node metastasis)and 10 cases of normal oral mucosa was detected by immunohistochemical SABC method. Microvessel density(MVD) in the samples was counted under microscope. Results:Ang-2 was detected in 28 out of 41(68.29%) cases of OSCC and 2 out of 10(20%) of normal oral mucosa(P