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To explore detection and adequacy evaluation of erythrocyte glutathione S transferase [GST] in hemodialysis patients on circular toxin levels, this paper divided 84cases of long-term hemodialysis end-stage patients into 33 cases of adequate hemodialysis group [spKtN>1.3] and 51 cases of inadequate hemodialysis group [spKtN<1.3] according to urea index value of unit chamber model [spKt/V]. Take the other 50 cases of healthy physical examination people for control group, compare and analyze related clinical and biochemical indexes differences of three groups. The level of hemodialysis group GST, creatinine, high sensitivity C-reactive protein [hs-CRP], transferrin saturation [TSAT], parathyroid hormone [PTH], interleukin-2,6,8 [IL-2,6,8] and tumor necrosis factor-a [TNF-a] was significantly higher than the control group [P<0.05], and GST, IL-2, 6, 8, TNF-a level of inadequate hemodialysis group was significantly higher than adequate hemodialysis group [P<0.05]. Pearson's relevant analysis showed that GST and spKt/V, IL-2, IL-6, IL-8, TNF-a have positive correlation [P<0.05] and had no correlation with creatinine, hs-CRP, TSAT, PHT [P>0.05]. There was 23patients spKtN>1.3 after adjusting the dialysis solution for 5lcases of inadequate hemodialysis patients, GST level after the adjustment was significantly lower than before the adjustment, but still higher than adequate dialysis eroup. It concludes that the maintenance of hemodialysis patients' level has certain relevance on spKt/V and associated inflammatory factors. Through the determination, GST can effectively response the adequate hemodialysis, which has a guiding significance on adjusting blood dialysis solution in clinic
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BACKGROUND: Estrogen exerts a negative regulatory role in adipogenic differentiation of adipose stem cells, but there is no report concerning estrogen effects on adipogenic differentiation of adipose stem cells from the adipose capsule of kidney after long-term cryopreservation. OBJECTIVE: To explore the impact of estrogen on adipogenic differentiation of fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule or those after long-term cryopreservation. METHODS: Passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule were divided into four groups, al of which were induced to adipogenic cells by induced fluid: fresh cells + 10-7 mol/L estrogen, cryopreserved cells + 10-7 mol/L estrogen, fresh cells and cryopreserved cells groups. Oil red O staining and adipogenic quantitative detection were performed at 14 days after induced adipogenic differentiation. RESULTS AND CONCLUSION: There were no differences in the morphology and arrangement between the cryopreserved and fresh cells. Both cryopreserved and fresh cells expressed CD29 and CD 44, but did not express CD31. Intracel ular lipid droplets were observed after adipogenic differentiation by oil red O staining, and the cells were positive for oil red O staining. The adipogenic volume comparison among the four groups was detected on day 14 after adipogenic differentiation, and the absorbance values showed significant difference between the fresh cells and fresh cells + estrogen groups, as wel as between the cryopreserved cells and cryopreserved cells + estrogen groups, but no difference between the fresh and cryopreserved cells groups. It is proved that low-dose estrogen can inhibit the adipogenic differentiation of long-term cryopreserved adipose-derived mesenchymal stem cells from the kidney adipose capsule; however, there is no significant difference between passage 3 long-term cryopreserved and fresh adipose-derived mesenchymal stem cells from the kidney adipose capsule in adipogenic differentiation.
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BACKGROUND: The stem cell transplantation achieved progress in treating acute kidney injury. However, cell transplantation in treating chronic renal damage, as well as the protection mechanism, remains poorly understood.OBJECTIVE: To observe the changes of urine protein and renal function, as well as the effects of bone marrow mononuclear cells on expression of transforming growth factor β1 (TGF-β1) and connective tissue growth factor (CTGF) in chronic renal damage rats.DESIGN, TIME AND SETTING: The randomized controlled animal experiment was performed at the Experimental Animal Center of General Hospital of Shenyang Military Command from May to October 2008.MATERIALS: Totally 30 female SD rats, with SPF grade, were randomly divided into the control, model and cell transplantation groups, with 10 animals in each group. Additional 10 SD rats were used to prepare bone marrow mononuclear cells.METHODS: Chronic renal damage models were prepared by modified hepatic resection, and 1.0 mL mononuclear cells were injected at 10 weeks after model preparation, with 1×10~8 cells per animal. Rats in the model group were injected same volume of physiological saline.MAIN OUTCOME MEASURES: The 24 hour urinary protein excretion quantity and renal function changes, histopathological change of renal tissues, as well as the expression of TGF-β1 and CTGF.protein excretion, urea nitrogen and creatinine levels (P < 0.01), which especially greater in the model group (P < 0.01 or P <expressions of TGF-β1 and CTGF were notably enhanced in the model and cell transplantation groups (P < 0.01), but the enhancement extent was larger in the model group (P < 0.01).CONCLUSION: Bone marrow mononuclear cells can decrease levels of urine protein excretion, blood urea nitrogen and creatinine, inhabit the TGF-β1 and CTGF expression, relief pathological changes, eventually, protect the kidney of subtotally nephrectomized rats.
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Objective To elevate the achievement rate of kidney biopsy puncture through the analysis of puncture angle choice of ultrasound guided automatic kidney biopsy in 518 patients. Methods Under the guidance of color Doppler ultrasound,using the 3.5MHz probe with probe holder,we performed kidney biopsy in the degrees of 40°(group X),45°(group Y),60°(group Z) and 70°(group P) with the horizontal plane. Resnlts The total achievement ratio is 100 %. The one time success rate in group Y and group Z is 100 % (group Y, 164/164 ; group Z, 186/186) ;group X is 4.8% (22/46) ;group P is 84.4% (103/122).Conclusion The automatic kidney biopsy an- gle under the guidance of ultrasound should be between 45° and 60° with the horizontal plane which is safe and has higher achievement ratio. In the meantime, complications can be avoided.
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Objective To investigate the leptin in continuous ambulatory peritoneal dialysis(CAPD)and its influen- cing factors.Methods 30 CAPD patients and 30 henhhy controls were enrolled in the study.Leptin was measured by ELISA.C reactive protein(CRP)by transmisson turbidmetry.Body mass index(BMI),fasting insulin(FINS), blood urea nitrogen(BUN),serum creatinine(Scr)etc were also determined.These patients were classified to two nutritional categories based on SGA normal nutrition and malnutrition.The relationship between serum leptin concentra- tion and these indices was analysed.Results Serum leptin levels in CAPD group was significantly higher than those of healthy control(P
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Objective To investigate the common causes of acute renal failure(ARF) in the elderly hospitalized patients and the effect of continuous peritoneal dialysis(PD) by bedside intubation.Methods The data of hospitalized patients with ARF including 28 elderly patients (mean age 68.2?9.6 yrs) and 13 adult patients (mean age 39.4?9.1 yrs) were studied.Of them,32 patients received PD by bedside intubation.The nosogenesis of ARF was analyzed and the effect of emergency PD by bedside cmpared between two groups.Results Among various causes,prerenal factors accounted for 32.1%,infection and wound of bad control 39.3%,unreasonable use of nephrotoxic drugs 14.3%,which totally accounted for 85.7%.Of 21 elderly patients (76%) receiving PD treatment,11 (52.4%) acquired completely remission,5 (23.8%) turned to chronic PD,the successful rate of intensive care being 81.0%,and the death rate being 19.1%.Conclusion Prerenal factors,infection,wound of bad and unreasonable use of nephrotoxic drugs are the common causes of ARF in the elderly hospitalized patients.PD by bedside intubation is safe,simple and effective.