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Objective To investigate the effects of interleukin?17A on kidney injury induced by paraquat(PQ). Methods Seventy?two ICR mice were randomly divided into 3 groups:NS,PQ,and PQ+Ab (n=24 for each). The PQ?poisoning model was established by administering a gavage of PQ solution;mice in the PQ+Ab group were then administereda dose of anti?IL?17A antibody 2 hours later by i.p. injection,whereas the NS group were administered a corresponding volume of normal saline instead.The mice were killed at 8,24,48,or 72 h to obtain renal tissues and serum. An enzyme?linked immunosorbent assay(ELISA)was used to determine serum IL?17A,serum creatinine(SCr),and blood urea nitrogen (BUN)levels.Chemical colorimetry was used to detect the viability of myeloperoxidase(MPO )in renal tissue,and hematoxylin?eosin(HE)stain?ing was used to observe the renal pathologic changes. Immunohistochemistry(IHC)and PCR were used to examine IL?17A expression in renal tis?sues. Results Serum IL?17A,renal tissue MPO viabilities,BUN,and SCr were increased in the PQ and PQ+Ab groups,compared to those in the NS group(P<0.01). However,the above?mentioned parameters were lower in the PQ+Ab group than in the PQ group(P<0.01). Conclusion IL?17A promotes mouse kidney injury induced by acute PQ?intoxication through activating and/or recruiting neutrophils;therefore,blockade IL?17A,with antibody can attenuate the injury.
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Objective To investigate the role of interleukin-17A (IL-17A) in acute paraquat (PQ)-induced lung injury in mice.Methods A total of 120 healthy SPF grade ICR male mice were randomly (random number) divided into three groups (n =40 in each):normal saline control group (NS),PQ poisoning group (PQ) and antibody neutralization group (PQ + Ab).Mice of PQ group and PQ + Ab group were given 5 mg/mL PQ by one gavage in a dosage of 25 mg/kg body weight,and 5 μg IL-17A neutralizing antibody intra-peritoneally administered immediately after PQ poisoning in PQ + Ab group;Equivalent volume of normal saline instead of PQ was given to mice of NS group.Six survival mice from each group were taken for experiment at 8 h,1 d,3 d,5 d,7 d after PQ poisoning:Wet to dry ratio (W/D) of lung was determined in mice of each group.HE staining of lung tissue was used to observe the histopathological changes under the light microscope and the pathological scores were graded;Serum interleukin-17A (IL-17A),interleukin-22 (IL-22),interleukin-6 (IL-6),transforming growth factor-β (TGF-β) were detected with enzyme linked immunosorbent assay (ELISA);Expression of interleukin-23 receptor (IL-23R) in lung tissue was determined with immunohistochemical;real-time fluorescence quantification PCR (qRT-PCR) was used to detect the expression of retinoic acid related solitary nuclear receptors' mRNA in lung tissue.Results After administration of PQ,W/D ratio increased (P ＜ 0.01),lung injury was observed in mice of PQ and PQ + Ab groups,levels of cytokines (IL-17A,IL-22,IL-6 and TGF-β) in serum elevated (P ＜0.05),and the expressions of IL-23R mRNA and RORγt mRNA increased (P＜0.01).But in PQ +Ab group,W/D ratio decreased (P ＜0.05),lung injury was alleviated,the levels of cytokines (IL-17A,IL-22,IL-6 and TGF-β) decreased (P ＜ 0.05),and the expressions of IL-23R mRNA and RORγt mRNA reduced (P ＜ 0.05).Conclusions Since IL-17A involves in the lung injury of the mice induced by acute paraquat poisoning,blockade of IL-17A significantly alleviates the acute lung injury in mice.
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Objective To investigate the correlation between the MRI morphology scoring of cartilage injury,the changes of T2 value and clinical manifestations in knee osteoarthritis (OA).Methods Ten healthy volunteers and 65 patients with OA were enrolled.The patients were graded according to the Western Ontario and McMaster University Osteoarthritis Index (WOMAC).Knees of all research participants underwent MRI scanning of sagittal 3D-WATSc and T2 mapping.Patients were further divided into group OA1,group OA2 and group OA3 based on Whole-Organ Magnetic Resonance Imaging Score (WORMS).The correlation between the WORMS scores,T2 values of the knee cartilages and the clinical WOMAC scores was analyzed.Results The WORMS scores of the cartilages were positively correlated with the total WOMAC scores,pain scores,stiffness scores and activity scores,with the correlation coefficient of 0.806,0.690,0.493 and 0.817,respectively (P＜0.05).The T2 values of the cartilages had a positive correlation with the total WOMAC scores,pain scores,stiffness scores and activity scores,with the correlation coefficient of 0.501,0.384,0.357 and 0.512,respectively (P＜0.05).Conclusion WORMS as MRI semi-quantitative evaluation index and T2 values as MRI quantitative evaluation index could reflect clinical manifestations of patients with knee OA to some extent.
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Objective To evaluate the prognosis of patients with sepsis in the emergency department using the modified CURB-65 score.Methods We retrospectively analyzed the clinical data of 143 patients with sepsis who were first diagnosed at the emergency department of the First Hospital of China Medical University (between January 2014 and January 2015),assessed their CURB-65 and sequential organ failure assessment (SOFA) scores,and modified the CURB-65 scoring system by adding some indexes of the prognosis of sepsis.We analyzed the prognostic value of each scoring systems in the diagnosis of sepsis using the receiver-operating characteristic curve.Results The modified CURB-65,CURB-65,and SOFA scores had independent abilities for early prediction of the prognosis of sepsis.The area under the curve and the Youden index of the modified CURB-65 score were highest,which are superior to the traditional CURB-65 and SOFA scores.Conclusion The modified CURB-65 score can predict the prognosis of sepsis in its early stage.In addition,the assessment method is simple and convenient;hence,it is useful for assessing the condition of patients with sepsis and providing an early treatment.
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Objective To analyse the multi-slice spiral CT(MSCT) findings of small cholangiocarcinoma of common bile duct.Methods 15 cases with pathologically verified small cholangiocarcinoma of common bile duct were undergone unenhanced and three-phase contrast-enhanced MSCT scan.The entire morphologic changes of common bile duct were analysed with curved planar reformation(CPR).Results The attenuation of tumor relative to pancreas was iso-density in all cases at plain CT scan,hypo-density in 10 cases,iso-density in 3 cases and hyper-density in 2 cases at arterial phase,hypo-density in 1 case,iso-density in 3 cases and hyper-density in 11 cases at portal phase,iso-density in 5 caaes and hyper-density in 10 cases at delayed phase.The focal wall thickening of common bile duct appeared as circular or eccentric in 13 cases,intraluminal nodule in 2 cases,common bile duct was narrowing sharply in 11 cases and ending abruptly in 4 cases at obstructive level.Conclusion The small cholangiocarcinoma of common bile duct is of certain characteristics at unenhanced and three-phase contrast-enhanced CT scan.
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Ten factors of the sexual dimorphism on the cranial breadth were analyzed by using the dis- criminant analysis in order to estimate the sex.Results showed that seven out of ten human skulls have sex difference(P
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Eleven measurements of human up palates from two hundred invividuals were analyzed by the discriminant analysis in order to determine sex.Statistical analysis of the up palate measurements on the sexual dimorphism showed that ten measurements have sex difference(P
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60 Chinese skulls(30 males and 30 females) from Liaoning province of the People's Republic of China were measured, By applying the multiplestepwise discriminant function, sexual diagnosis of the maxillary, frontal, occipital and parietal bone were carried out, 12 discriminant equation for sexual diagnosis have been obtained,The diseriminant rate of equations with accuracy tests on the same series of crania results in 66.7～93.3% of the cases analyzcd