Preventive effect of low-dose aspirin on preeclampsia occured in preeclampsia high-risk pregnant women and its mechanism / 吉林大学学报(医学版)

Objective: To observe the preventive effect of low-dose aspirin (LDA) in the preeclampsia (PE) high-risk pregnant women, and to elucidate the feasibility and clinical value of LDA in preventing PE. Methods: A total of 112 PE high-risk pregnant women with 16-22 gestational weeks were selected and randomly divided into control group (n = 5 8) and observation group (n = 5 4). The patients in control group were given placebo treatment, the patients in observation group were given 75 mg • d-1 LDA before going to bed until delivery. The incidence of PE, gestational age of onset, gestational age of delivery, neonatal birth weight, delivery mode, neonatal outcomes of the patients in two groups were observed. The patients in two groups were divided in to PE (+) (with PE) group and PE (-) (without PE) group according to whether PE occured or not. The coagulation function and the platelet parameters of the patients in two groups were detected; the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), thromboxane A2 (TXA2), P-selectin in placenta tissue of the patients in two groups were detected by ELISA method. Results: Compared with control group, the incidence of PE and the incidence of premature delivery of the patients in observation group were significantly decreased (P < 0. 05); the gestational age of delivery of the patients in observation group were prolonged (P < 0. 05), the neonatal birth weight was increased (P < 0. 01), and the full-term birth rate was increased (P < 0. 01). Compared with the PE (+) and PE (-) patients in control group, the prothrombin time (PT) and activated partial thromboplastin time (APTT) of the PE (-) patients in observation group were increased (P < 0. 01), and the PT of the PE (+) patients in observation group was decreased (P < 0. 01); the levels of prothrombin activity (P T A), fibrinogen (FIB) and D-dimer (D-D) of the PE (+) and PE (-) patients in observation group were decreased. Compared with control group, the levels of IL-6, TNF-αa, COX-2, TXA2 and P-selectin in placenta tissue of the patients in observation group were decreased significantly (P < 0. 01). Conclusion: LDA treatment within 16 - 22 weeks of gestation can effectively prevent the occurrence of PE in the PE high-risk pregnant women, its mechanism is related to improving the coagulation function and inhibiting the protein expressions of inflammatory factors in the PE high-risk pregnant women.

Characteristics and prognosis of interrupted inferior vena cava with azygous continuation / 中华妇产科杂志

Objective To explore the clinical characteristics of interrupted of the inferior vena cava with azygous continuation and the prognosis.Methods Retrospective analysis of 21 fetuses diagnosed with interrupted inferior vena cava with azygous continuation among 28 567 pregnant women who underwent routine ultrasound scan.The clinical data,ultrasonographic features,genetic information and prognosis were collected. Results Interrupted of the inferior vena cava with azygous continuation occurred in 21(0.07%, 21/28 567)of 28 567 patients.Three fetuses(14%,3/21)complicated with heart and extracardiac malformations, including endocardiac cushion defect,single atrium and single ventricle,double superior vena cava,dextrocardia, asplenia syndrome,visceral heterotaxy,duodenal atresia;six fetuses(29%,6/21)were associated with cardiac anomalies, such as hypoplastic left heart syndrome, double outlet right ventricle, pulmonary stenosis, ventricular septal defect,persistent left superior vena cava,endocardiac cushion defect and transposition of the great arteries;six cases(29%,6/21)were only combined with extracardiac malformations,includingasplenia syndrome, visceral heterotaxy, duodenal atresia. Three fetuses (14%,3/21) were nonorganic abnormalities included thickening of the right ventricle wall, fetal bradycardia, pericardial effusion, hydrops abdominis, increased peak systolic velocity/end diastolic velocity and single umbilical artery.Three fetuses(14%,3/21) were isolated interrupted inferior vena cava with azygous continuation,but without other anomalies and 2 of them had normal fetal karyotype.Five cases(24%,5/21)were successfully vaginal delivery,1 case(5%,1/21) had cesarean section. After 12-40 months follow-up, we didn′t obeserve obviously abnormality, nor any chromosomal abnormality.Ten patients(48%,10/21)opted for termination of the pregnancy and the autopsies were not done.Five cases(24%,5/21)were lost to follow up.Conclusions Interrupted inferior vena cava with azygous continuation are associated with cardiovascular and extracardiac anomalies, cardiac malformation and visceral heterotaxy are the most common anomalies. Visceral heterotaxy should be considered and fetal karyotype should be suggested. In the cases of isolated interrupted inferior vena cava with azygous continuation and normal karyotype,the outcome is favorable.

Improvement of acidic polysaccharose of Schisandra Chinensis on learning and memory functions of Alzheimer's disease model mice / 吉林大学学报(医学版)

Objective:To observe the improvement of acidic polysaccharose of Schisandrae Chinensis (SCP-A) on the learning and memory functions of Alzheimer's disease (AD)model mice induced by Aβ25-35 ,and to clarify the related mechanisms. Methods:A total of 75 male C57BL mice were randomly divided into control group (injected with saline in intracerebroventricular,given distilled water intragastrically),model group (injected with Aβ25-35 intracerebroventricularly,given distilled water intragastrically),and 5,10,20 mg·kg-1 SCP-A groups (injected with Aβ25-35 intracerebroventricularly,given SCP-A intragastrically) (n = 15).The agents were administered once daily for 14 d,in which Aβ25-35 was injected intracerebroventricularly on the 8th day.On the 15th day after administration,step-through test and Morris water maze test were used to detect the escape latency,number of errors,and time of finding platform,number of passing platforms,dwell time in target quadrant of the mice;Western blotting method was used to detected the levels of Tau,GSK-3β and their phosphorylated proteins in hippocampus tissue of the mice.Results:The step-through test results showed that compared with control group, the escape latency of the mice in model group was obviously shortened and the number of errors was significantly increased (P <0.01);compared with model group,the escape latencies of the mice in 10 and 20 mg·kg-1 SCP-A groups were significantly prolonged (P <0.01),the number of errors was significantly decreased (P <0.05 or P <0.01).The Morris water maze test results showed that compared with control group,the time of finding platform of the mice in model group was significantly prolonged,the number of passing platform and dwell time in target quadrant were significantly reduced (P <0.01);compared with model group,the time of finding platform of the mice in 10 and 20 mg· kg-1 SCP-A groups was significantly shortened (P < 0.01),and the number of passing platform and the dwell time in target quadrant were significantly increased (P <0.01).The Western blotting results showed that compared with model group,the expression levels of phosphorylated protein Tau Ser199,Tau Ser396 and Tau Ser404 in hippocampus of the mice in 20 mg·kg-1 SCP-A group were significantly decreased (P <0.05), and the expression level of GSK-3βwas significantly decreased (P <0.01);the expression level of phosphorylated protein GSK-3βTyr216 was significantly decreased,and the expression level of phosphorylated protein GSK-3βSer9 was significantly increased (P <0.05).Conclusion:SCP-A has an anti-AD effect,which is related to regulating the activity of GSK-3βto reduce the level of phosphorylated protein Tau in the hippocampus tissue of the mice.

Factors and prevention measures of postoperative infection in laryngeal cancer patients / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To investigate the factors of postoperative infection in patients with laryngeal cancer,and propose preventive measures,to provide a theoretical basis for the control of hospital infection.@*METHOD@#Here we conducted an epidemiological study of 259 patients undergoing surgery in our hospital laryngeal surgery, and analyzed the influencing factors of postoperative infection.@*RESULT@#Among 259 patients, 18 cases (6.95%) patients had postoperative infection; multifactor by Logistic regression analysis showed that there was a correlation about patients postoperative infection was associated with age (OR = 20.694, P < 0.05), preoperative radiotherapy (OR = 19.719, P < 0.05), hospitalization (OR = 10.679, P < 0.05 ), operative time (OR = 14.387, P < 0.05), type of surgery (OR = 4.771, P < 0.05), tracheostomy (OR = 55.795, P < 0.05), antibiotic use (OR = 12. 486, P < 0.05) and postoperative nutritional support (OR = 7.928, P < 0.05).@*CONCLUSION@#Strict implementation of the treatment of laryngeal surgery aseptic,shorter hospital stay and operative time, strengthen the operating room disinfection, perioperative, and postoperative care of patients with nutrition, rational use of antibiotics,planned radiotherapy for advanced tumor, are the key measure to reduce postoperative infection rate of laryngeal.

Effects of enalaprilat on proliferation of cardiac fibroblasts and molecular mechanism / 吉林大学学报(医学版)

Objective To observe the effects of ACE inhibitor enalaprilat(Ena) and protein kinase C(PKC) inhibitor chelerythrine(Chele)on proliferation,collagen Ⅰ,cell cycle,PKC and cyclinD1 protein expression of neonatal cardiac fibroblasts(CFb) and to probe its molecular mechanism.Methods CFb of neonatal Wistar rats were divided into control group,AngⅡ group,Chele+AngⅡ group,Chele+AngⅡ+Ena group and AngⅡ+Ena group.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,immunocytochemical staining(IC) was used to measure collagen Ⅰ content,Western blotting and flow cytometry were used to detect PKC,cyclinD1 and cell cycle respectively.Results Compared with AngⅡ group,the MTT value decreased dramatically(P

Effects of Enalaprilat on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts / 吉林大学学报(医学版)

Objective To investigate the effects of angiotensin Ⅰ-converting enzyme inhibitor Enalaprilat(Ena) on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts(CFb)and to probe its anticardiac fibrosis mechanism.Methods The cultured neonatal Wistar rat CFb were divided into control group,model group and three doses of Ena groups.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,flow cytometry was used to measure cell cycle,nitric acid reductase method and spectrophotometry were used to detect the NO contents and NOS activity respectively with Ena.Results Ena could inhibit CFb proliferation induced by AngⅡ,there were significant differences of MTT values between model group and Ena groups after treated for 24,48 and 72 h(P

Technical progress of the ECT device / 医疗卫生装备

This paper introduces the technical progress of the emission computed tomography (ECT) device in these years. As the latest technical advance and applications in nuclear medicine imaging, the features, performances and applications of CTs of dual-head coincidence (DHC) single photon emission computed tomography (SPECT) and positron emission computed tomography (PET) are discussed.

Research of enalapril on rat myocardial fibrosis and its mechanism / 中国药理学通报

Aim To investigate the effects of enalapril(Ena) on cardiac fibrosis induced by isoprenaline(Iso) and to explore its mechanism.Methods Effect of Ena and captopril(Cap) on collagen content,HW/BW,LVI,hydroxyprolnie(Hyp) level,and TGF-?1 protein expression was observed with IH and WB in rats induced by Iso subcutaneous injection.Results Different doses of Ena could decrease HW/BW,LVI and Hyp level dramatically,and decrease TGF-?1 protein expression which was closely related to myocardial fibrosis(MF).Conclusion Enalapril can inhibit TGF-?1 protein expression,by which inhibit myocardial fibrosis.

Research of enalaprilat on neonatal rat cardiac fibroblasts proliferation and its mechanism / 中国药理学通报

Aim To investigate the effects of angiotensin-converting enzyme inhibitor Ena on proliferation and cell cycle protein of cardiac fibroblasts and to explore the mechanism of Ena on cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,collagen synthesis was observed by hydroxyproline concentration method,flow cytometry,immunofluorescenic and Western blot was used to measure cell cycle and CKI p27kip1 with Ena.Results Ena decreased MTT value and collagen synthesis dramatically;Ena increased phase G0/G1 and decreased phase S percentage ratio in cell cycle;Ena enhanced p27kip1 protein expression in a dose-dependent manner.Conclusion The antiproliferative effects of Ena on CFb can be attributed to upregulating CKI p27kip1 protein expression.

Enalaprilat inhibited the proliferation of Neonatal rat Cardiac Fibroblasts via modulation of endothelin-1 and nitric oxide production / 中国药理学通报

Aim To investigate the effects of angiotensinI-converting enzyme inhibitor enalaprilat on proliferation,Endothelin-1 and Nitric oxide System of neonatal cardiac fibroblasts and to probe its mechanism of inhibiting cardiac fibrosis.Methods CFb was isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,HYP method was used to test the collagen synthesis;FCM for cell cycle;Nitric acid reductase method,radio-immunological method was used to detect NO content and ET-1 activity respectively.Results Ena decreased contents of MTT and HYP dramatically.The percentage ratio of phase G0/G1 was increaseded and the percentage radio of phase S was decreased.NO content was increased and ET-1 activity was dropped in a dose and time-dependent manner.Conclusion The anti-proliferative effects of Ena on CFb can be attributed to dropping endothelin-1 activity and enhancing Nitric oxide.