RESUMEN
Vitamin D is one of the fat soluble vitamins, it has a great role in calcium and phosphorus metabolism. The effect of hypervitaminosis was studied on 75 adult female albino rats. The rats were divided into three groups, control, treated and recovered group. The control group consisted of 15 rats were divided in a groups 5 rats each and were sacrified at beginning, after one month and after two months of the beginning of the experiment without injection of vitamin D. The rats of treated groups were divided into 3 groups A, B and C [10 rats each], the treated group A received 2 doses of vitamin D [12000 IU] intraperitoneally 2 weeks interval, treated group B received 3 doses, 10 days interval and treated group C received 4 doses one week interval. All rats were sacrified after one week from last injected dose. The rats of recovered group were divided into 3 groups A, B and C [10 rats each]. Each group received the same doses as in treated groups A, B, C respectively, but all rats were left 4 weeks after last injected dose before sacrifice. The ovaries of all rats were extracted, cut in sections and stained with Haemotoxylin and Eosin to visualize the general architecture of the ovaries and with modified von Kossa for detection of calcium. The rats of the treated groups showed multiple changes in the form of decreased food consumption and decreased body weight. Histologically, the ovaries showed decreased total number of all types of follicles [primordial, primary, secondary and Graaffian follicles] and corpora lutea, dilated blood vessels, multiple areas of hemorrhage in the corpora lutea and their vaculations were decreased. The secondary and the Graaffian follicles showed distortion of their granulose cells and decreased number and size. All these changes were dose dependent, increasing with increased number of the total doses received. The recovered groups showed improvement of different degrees, increased markedly in the recovered group A, to a lesser extent in the recovered group B, and very minimal in the recovered group C. It is recommended that the proper manner of injection of vitamin D in the treatment of different types of malignancies is to give two doses separated by two weeks intervals and then the patient is left for two weeks to avoid any permanent harmful effects on the ovary and other tissues