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Objective:To explore the prognostic value of tumor deposits (TD) by number and anatomical distribution in gastric cancer (GC) patients without lymph node metastasis.Methods:From Aug 2012 to Aug 2018 all 91 GC patients undergoing radical gastrectomy and without nodal metastasis at Yijishan Hospital of Wannan Medical College were enrolled in this study. Patients were divided into L1, L2, and L3 groups according to the number of TD and into Q1 and Q2 groups according to the anatomical regions of the TD.Results:The 3-year overall survival (OS) rates of groups L1, L2, and L3 were 58.9%, 52.1%, and 31.5%, respectively ( χ2=9.769, P=0.008). The 3-year OS rates of groups Q1 and Q2 were 58.9% and 7.1% ( χ2=46.310, P<0.001). The number of TD, their distribution, neural invasion, vascular invasion, tumor size, and pT stage were all related to prognosis by univariate analysis (all P<0.05). Tumor size>4 cm ( HR=2.460, 95% CI:1.307-4.629, P=0.005), distribution of TD (non-perigastric)( HR=3.959, 95% CI:2.077-7.545, P<0.001), neural invasion ( HR=4.299,95% CI:1.953-9.461, P<0.001), and pT 4 stage ( HR=2.283, 95% CI:1.250-4.171, P=0.007) were independent risk factors for prognosis by multivariate analysis. Conclusion:The distribution of TD (non-perigastric) is an independent risk factor for poor prognosis in gastric cancer patients after radical gastrectomy and with negative lymph node metastasis.
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Objective To examine the effects of aerobic exercise on muscle morphology,oxidation resistance,energy metabolism,mitochondrial biogenesis-related signal transduction pathway molecules,and energy metabolism-related enzyme proteins expression in aged rats and to explore the molecular mechanisms of skeletal muscle aging and aerobic exercise intervention.Methods Male Sprague Dawley rats were divided into a normal group,a model group,and an exercise group,with the latter two groups receiving a subcutaneous injection of 10％ D-galactose(0.1 mg · g-1 · d-1)for 10 weeks.Then aerobic and anaerobic exercise capacity of each group were tested by treadmill;the cross-sectional area of rats' gastrocnemius was examined via light microscopy;levels of serum superoxide dismutase(SOD) activity and malondialdehyde(MDA) were determined by spectrophotometry;serumβ Galase levels were tested by enzyme-linked immunosorbent assay (ELISA);levels of adenosine triphosphate (ATP),adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in gastrocnemius tissue were measured by high performance liquid chromatography(HPLC) ultraviolet detection;expression levels of mitochondrial biosynthesis-related signal transduction molecules(such as p38MAPK,CaMK Ⅱ,PGC-1α,MEF2C,COXⅣ and ATPase) were tested by real-time RT-PCR.Results Compared with the normal group,the model group's cross-sectional area of muscle fibers,serum SOD activity,ATP content,and EC values all markedly decreased,while serum MDA,β Galase,AMP content in skeletal muscle significantly increased(all P ＜0.05).Levels of mRNA expression of CaMK Ⅱ,MEF2C,ATPascβ,COXIV,and PCG-1 alpha in model rats notably decreased(all P＜0.05),whereas mRNA expression levels of P38MAPK significantly increased(P＜0.05).Compared with the model group,aerobic exercise increased the cross-sectional area of muscle fibers(P＜0.01)and serum SOD activity (P ＜ 0.05),reduced serum MDA content (P ＜ 0.01),clearly increased CaMK Ⅱ and ATPaseβ％ mRNA expression(both P ＜ 0.01),and reduced the expression of P38MAPK mRNA (P ＜ 0.01).Conclusions Aerobic exercise can improve the activity of SOD,reduce the accumulation of oxygen free radicals,resist oxidation damage,correct the abnormal activity of P38MAPK,and promote the expression of mitochondrial biosynthesis-related signal molecules,such as CaMK Ⅱ,PCG-1 alpha,and MEF2C,thus boosting the expression and activity of energy metabolism enzyme protein COX Ⅳ,ATPase,promoting energy metabolism,improving the production of ATP,and slowing down the ageing process of the body and skeletal muscle.
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Objective] To discuss application of KNN-kernel clustering methods for diarrhea patients serum immune indexes detection data classification and diagnosis of applicability and clinical significance. [Methods] To reveal the applicability and clinical signnificance of KNN-kernel function clustering method in the diagnosis of serun immune index. In this research, the KNNCLUST algorithm is used to program the serum immune index data of 74 patients with diarrhea by Matlab software. [Results] 74 patients were divided into 5 categories by cluster analysis. The patients with diarrhea were divided into rotavirus negative and positive class, and the patients were further subdivided, especially the three early rotavirus tests were negative but later confirmed positive and were clustered into one group. [Conclusions] This can be seen that the KNN-kernel clustering method is helpful for early screening of rotavirus infection, practical clinical significance on the early treatment of disease.
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Objective To compare the differences between TCM constitution test and fitness test of university students from different aspects;To prove that bringing TCM constitution test into fitness test of university students is feasible. Methods Comparative method was used to compare the differences between TCM constitution test and fitness test of university students from the aspects of basic theory, test content, test methods, and interventions. Results There were differences on the aspects of basic theory, test content, test methods, and interventions between TCM constitution teat and fitness test of university students. The test population and test aim were the same. TCM constitution test could comprehensively test physical, psychological, and social adaptability of university students. The individualized program of health maintenance could be given according to TCM constitutions after TCM constitution test. But this test is subjective. The fitness test of university students mainly focus on measuring physical health and the test method is objective. Conclusion TCM constitution test can be brought into current fitness test of university students to test their healthy condition more comprehensively and accurately. The individualized program of health maintenance can be offered after the test to promote health of university students.
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Objective To investigate the effect of 5-aza-2'deoxycytidine (5-Aza-CdR) on proliferation and expression of RASSF1 A gene in human ovarian cancer cell line SKOV3 and 3AO.Methods SKOV3 and 3AO cells were treated with different concentrations (0.5,5,50 μmol/L) of DNA methyltransferase inhibitor 5-Aza-CdR.RT-PCR and Western Blot were adopted to detect expression of mRNA and protein of RASSF1A gene before and after treatment with 5-Aza-CdR respectively.Results Compared with control group,when the 5-Aza-CdR concentration was 0.5,5,50 μmol/L after drug treatment,human ovarian cancer cells could significantly inhibit tumor cell growth; SKOV3 and 3AO cells in control group were observed weaker expression of RASSF1A mRNA.After treated with 5-Aza-CdR,the expressions of RASSF1A mRNA were observed increased with the increase of the drug concentration.After treated with different concentration of 5-Aza-CdR,the expressions of RASSF1A mRNA treated with 0.5 μmol/L 5-Aza-cdR was lower than those treated with 5 and 50 μmol/L 5-Aza-cdR (t =-8.866,P =0.01 ; t =-12.256,P =0.000).However,expressions of RASSF1A mRNA treated with 5 and 50 μmol/L 5-Aza-cdR respectively showed no statistical significance (t =0.431,P =0.689).Expressions of RASSF1A protein treated with 0.5 μmol/L 5-Aza-cdR and 5 μmol/L 5-Aza-cdR didn't show statistically significant (t =-1.586,P =0.188).Conclusion Expressions of RASSF1A mRNA and protein in SKOV3 and 3AO cells were evidently enhanced.As one kind of methyltransferases inhibitors,5-Aza-CdR can inhibit ovarian cancer cell line SKOV3,3AO growth through the RASSF1A promoter methylation,and thus promote their apoptosis.
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Objective To investigate the effects of catheter balloon dilatation on patients with dysphagia caused by neurogenic cricopharyngeal achalasia.Methods Thirty-two patients with dysphagia caused by neurogenic cricopharyngeal achalasia were divided randomly into a treatment group (n =16) and a control group (n =16).The treatment group received catheter balloon dilatation therapy and routine dysphagia rehabilitation training; the control group was treated with routine dysphagia rehabilitation training only.Both groups were tested before treatment,after 3 weeks of treatment (after treatment) and at 6 months after treatment (follow-up).All cases were evaluated using Saitoh's 7-grade swallowing assessment and videofluoroscopy.Results After treatment,5 cases in the treatment group had been cured (31％) and 8 cases showed marked effectiveness (50％) ; in the control group,1 case was cured (7％) and 5 cases showed marked effectiveness (31％).These differences were statistically significant.At follow-up,5 cases in the treatment group had been cured (31％) and 9 cases showed marked effectiveness (56％) ; for the control group,1 case was cured (7％) and 6 cased showed marked effectiveness (38％).Those differences were also statistically significant.After treatment and also at follow-up,the average time for a bolus to pass the pharynx in the treatment group was significantly shorter than that in the control group.Conclusion Catheter balloon dilatation therapy is effective for treating neurogenic cricopharyngeal achalasia in both the short and long term.
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According to request of the socialism outlook for honor and shame to university students,this article elaborated the principles,connotation and implementation of honesty evaluation system so as to make a beneficial exploration for the honesty education of university students.
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Objective To investigate the expression of MMP-9 genes in primary hepatocellular carcinoma(HCC).Methods RT-PCR was used to detect MMP-9 mRNA in surgically extracted specimens from 28 patients with HCC.Results The expression of MMP-9 mRNA was significantly higher in tumor than in corresponding nontumoral tissue(P0.05).Meanwhile,the expression of MMP-9 mRNA in HCC was significantly correlated to the existence of portal vein tumor thrombus(P