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Journal of Southern Medical University ; (12): 1073-1075, 2015.
Artículo en Chino | WPRIM | ID: wpr-333680

RESUMEN

<p><b>OBJECTIVE</b>To investigate the effect of anti-miR-145 on human airway smooth muscle cell (HASMC) proliferation and osteopontin systhesis in vitro and explore the mechanisms.</p><p><b>METHODS</b>HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting.</p><p><b>RESULTS</b>Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs (P<0.05 or <0.01), and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis (P<0.01).</p><p><b>CONCLUSION</b>Anti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.</p>


Asunto(s)
Humanos , Remodelación de las Vías Aéreas (Respiratorias) , Apoptosis , Proliferación Celular , Células Cultivadas , MicroARNs , Miocitos del Músculo Liso , Osteopontina , Sistema Respiratorio , Biología Celular
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