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1.
China Journal of Chinese Materia Medica ; (24): 4223-4228, 2015.
Artículo en Chino | WPRIM | ID: wpr-279257

RESUMEN

A comparable study were carried out by determination of trace elements on five marine-derived shell traditional Chinese medicine (TCM) (Ostreae Concha, Haliotidis Concha, Margaritifera Concha, Meretricis Concha, and Arcae Concha), which were recorded in the Chinese Pharmacopoeia (2010 version). Seven trace elements in 51 batches of this type of shell TCM were analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS), combined with principal component analysis (PCA) methods. The content of element Se, which exhibited significant differences among different drugs, could be used as a key element to distinguish this type of drugs. Meanwhile, the contents of elements Co, Cu, Mo, and Ba in Haliotidis Concha, Co and As in Margaritifera Concha, Mo and As in Meretricis Concha, Mo, As, and Ba in Arcae Concha, and Zn in Meretricis Concha were relatively stable. In the PCA plot, Arcae Concha and Meretricis Concha could be efficiently distinguished from Ostreae Concha together with Haliotidis Concha, and Margaritifera Concha. The results also showed a correlation with their medicinal function. In conclusion, trace elements in marine-derived shell TCM could not be neglected for their quality control.


Asunto(s)
Animales , Exoesqueleto , Química , Organismos Acuáticos , Química , Bivalvos , Química , Espectrometría de Masas , Medicina Tradicional China , Oligoelementos
2.
China Journal of Chinese Materia Medica ; (24): 3291-3294, 2014.
Artículo en Chino | WPRIM | ID: wpr-244578

RESUMEN

The identification of five marine-derived shell traditional Chinese medicine (TCM) recorded in the Chinese Pharmacopoeia were studied. Using near infrared technology (NIR) combined with principal component analysis (PCA) methods, Ostreae Concha, Haliotidis Concha, and Margaritifera Concha could be efficiently distinguished from Meretricis Concha together with Arcae Concha. In the first principal components, Ostreae Concha exhibited obvious differences with high loadings in 4 236, 5 263, 7 142 cm(-1) concerning to the contents of CaCO3 and H2O in the samples. Arcae Concha and Meretricis Concha displayed significant differences with others in the second principal components, which can be illustrated by high loadings in 5 000 -4 430 cm(-1) areas. It is indicated that the second principal components might be related to organics which contained NH and CH groups, for example proteins. Meanwhile, our data showed a correlation between the function of these shell TCM and their distribution in the PCA plot. These results suggested that organic components in marine-derived shell TCM could not be neglected for their quality control.


Asunto(s)
Animales , Exoesqueleto , Química , Carbonato de Calcio , Medicina Tradicional China , Métodos , Moluscos , Química , Clasificación , Análisis de Componente Principal , Agua de Mar , Especificidad de la Especie , Espectroscopía Infrarroja Corta , Métodos
3.
Journal of Central South University(Medical Sciences) ; (12): 553-557, 2005.
Artículo en Chino | WPRIM | ID: wpr-813507

RESUMEN

OBJECTIVE@#To investigate the tumor-suppression effect of PA combined with GM-CSF, TNF-alpha and IL-4 on cord blood mononuclear cells (CBMC).@*METHODS@#The mononuclear cells were isolated from human umbilical cord blood and cultured with polyacttin A (PA), GM-CSF + TNF-alpha + IL-4 (GTI), and GTI + PA (GTIP) respectively. Six days later, surface antigen expression of the cultured cells, including CD1a and CD83, which were the specialized markers of dendritic cell (DC), were analyzed by immunohistochemistry technique. The CBMC were cultured with GTI for 24 h to enhance DC, then were added apoptotic/necrotic Hela/HepG2 tumor cells, and finally PA was co-cultured. The antitumor cytotoxicity of CBMC was measured by MTT assay.@*RESULTS@#After the culture, CD1a and CD83 positive cell rates of the PA group inreased significantly, reaching (19.63 +/- 3.61)%, (9.28 +/- 4.31) % respectively, much higher than that of the control, but lower than that of the GTI group. The killing rate to the tumor cells of CBMC cultured with GTIP increased remarkably, much higher than the control, GTI and PA groups. After tumor antigens were added to the CBMC of GTIP group (GTIP + Tc), the killing rate increased.@*CONCLUSION@#PA not only promotes the proliferation and maturation of cord blood derived DC, but also improves the tumor-suppression effect of CBMC cultured with GTI.


Asunto(s)
Humanos , Antígenos CD , Genética , Antígenos CD1 , Genética , Células Cultivadas , Sangre Fetal , Biología Celular , Glicopéptidos , Farmacología , Factor Estimulante de Colonias de Granulocitos y Macrófagos , Farmacología , Células HeLa , Inmunoglobulinas , Genética , Inmunoterapia , Interleucina-4 , Farmacología , Leucocitos Mononucleares , Alergia e Inmunología , Neoplasias Hepáticas , Patología , Terapéutica , Glicoproteínas de Membrana , Genética , Neoplasias , Terapéutica , Células Tumorales Cultivadas , Factor de Necrosis Tumoral alfa , Farmacología
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