1.
Journal of Shanghai Jiaotong University(Medical Science)
;
(6): 20-21, 2001.
Artículo
en Chino
| WPRIM
| ID: wpr-635220
RESUMEN
Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. MethodsThe mobile phase was consisting of methanol-1% triethylamine(57:43) and omeprazole (OMZ) as internal standard. The detection was carried out with an ultraviolet detector operated at 235nm. ResultsThere was linearity over the range of 2. 056~41.12 ug/ml in the humor aquosus, r=0.9997. The average recovery of ATST was 94.58 %. Intra-day and in- ter-day RSD were less than 5 % and 10 % ( n = 5), respectively. Conclusion The method is reliable. It can be used for the study on the pharmacokinetics of ATST.