RGD peptide-modified chitosan as a gene carrier of implant surface / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study is conducted to explore new methods to perform surface biomodification of titanium implants and improve osteogenic efficiency.</p><p><b>METHODS</b>An RGD peptide and chitosan (CS) were combined by acylation reaction, forming RGD-CS. An RGD-CS/pDNA complex was subsequently prepared using a complex coacervation method and grafted on a pure titanium surface after physical and biochemical treatments were performed. The chemical structural characteristics of RGD-CS were evaluated using an infrared spectrometer and an elemental analyzer. The shape of this complex was then assessed by gel electrophoresis combined with atomic force microscopy. The grafting effect of this complex on the titanium surface was detected by EB staining.</p><p><b>RESULTS</b>CS and RGD peptides were coupled by an amide bond. The RGD-CS/pDNA complex was completely composited at N/P > or = 2. Atomic force microscopy results showed that the morphology of this complex was mainly spherical. EB staining experiments showed that this complex was successfully grafted on the titanium plate.</p><p><b>CONCLUSION</b>RGD peptide-modified CS can be used as a titanium implant surface plasmid package carrier of pDNA.</p>

Effects of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of cetylpyridinium chloride buccal tablets on halitosis induced by oral conditions.</p><p><b>METHODS</b>With Porphyromonas gingivalis, Prevotella intermedia and Fusobacterium nucleatum as the testing bacteria, the minimal inhibitory concentration (MIC) of cetylpyridinium chloride buccal tablets was determined using minute amount serial dilution test. The production of volatile sulfur compounds (VSCs) was measured using sulfide detector halimeter in the anaerobic bacteria culture at 4 and 8 h after addition of the tablets. The effect of the tablets in suppressing odor production by mouth-borne halitosis bacteria was assessed using cysteine challenge test in healthy volunteers, and the effectiveness was evaluated by measuring the reduction in VSCs production and the duration of the effect.</p><p><b>RESULTS</b>Cetylpyridinium chloride buccal tablets inhibited the growth of all the 3 bacteria. The tablets obviously inhibited VSCs production by the 3 bacteria with a effect similar to chlorhexidine. Compared with distilled water gargle, the buccal tablets significantly reduced cysteine-induced VSCs production level in the healthy volunteers (P<0.05), and the effect lasted for 230 min.</p><p><b>CONCLUSION</b>Cetylpyridinium chloride tablets can obviously suppress bacteria responsible for oral halitosis and produce good effects in the treatment of halitosis induced by oral conditions.</p>