RESUMEN
Objective@#To introduce the application of free-style perforator flap based on aesthetic units to repair facial defect after tumor resection.@*Methods@#By following the concept of free-style perforator flap and the principle of facial aesthetic unit, the design of a free-style perforator flap allowed over any nearby cutaneous vessel chosen purely on the characteristics of its Doppler signal. Conventional knowledge of anatomical landmarks and possible vascular variations were less relevant. A greater freedom in flap selection was gained to recover defect in different forms such as rotation flap, advanced flap and propeller flap, which were all based on free-style perforators. The flap size ranged from 1.5 cm×1.0 cm to 12.0 cm×6.0 cm with the perforator diameter of 0.3-3.0 mm in pedicle, and some of the pedicles are "perforator clusters" .@*Results@#A total of 72 cases underwent surgery, and 68 cases survived completely with satisfactory appearance. 1 case healed two weeks later through dressing due to undesired healing, which result ed from high tension secondary postoperative blooding. 3 cases healed in a delay due to congestion and gained acupuncture treat.@*Conclusions@#The free-style perforator flap, which depended on Doppler-discerned perforator and facial aesthetic unit, represents safe, reliable and versatile for repairing facial defect after extended resection, and it not only offers a greater freedom in flap selection but also provides good aesthetic result.
RESUMEN
The growth of P. cruentum when added organic carbon source, organic nitrogen source and group B vitamin into medium were investigated in the present work. Results showed that glucose promoted growth rate observably. When addedZ% (W/V)glucose into the medium, the growth rate was doubled and biomass increased 92.6%to that of control after incubated 10 days . organic nitrogen source restrained the growth or harmed to P. cruentum. Vitamin Be and B12 also promoted the growth rate.
RESUMEN
The growth of P.cruentum when added organic carbon source. organic nitrogen source and group B vitamin into medium were investigated in the present work. Results showed that glucose promoted growth rate observably. When added2%(W/V)glucose into the medium, the growth rate was doubled and biomass increased 92 6%to that of control after incubated 10 days .organic nitrogen source restrained the growth or harmed to P.cruentum. Vitamin B 2 and B 12 also promoted the growth rate.