Detection of mycoplasma infection in sheep and goats by using PCR technique, with the associated pathological lesions

Sheep and goats are important agriculture animals in Egypt. Mycoplasma is the most frequent causes of morbidity, mortality and economic losses associated with sheep and goats. Three mycoplasma species were obtained in this study from sheep and goats at Cairo abattoirs. These mycoplasmas were found either as mixed or single infections and identified by biochemical and growth inhibition / [GI] tests. Mycoplasma isolates were classified into three biochemical groups: group [1] contained 12 isolates [33.33%] from sheep and 6 isolates [23.1%] from goats [M. agalactiae]. Group [2] contained 12 isolates [33.33%] from sheep and 10 isolates [38.5%] from goats [M. ovipneumoniae]. Group [3] contained 12 isolates [33.33%] from sheep and 10 isolates [38.5%] from goats [M.arginini]. PCR technique was found to be much faster than conventional microbiological techniques for isolation and identification and results could be obtained within five hours. In our study, six field isolates of M. agalactiae were tested by PCR, the results had confirmed that the field isolates were M. agalactiae by the presence of specific single band at 360 bp in each of field isolates and M. agalactiae reference stain. Also PCR amplification of M. ovipneumoniae strains gave a characteristic band at 1070 bp. Six field isolates were tested for detection of M. arginini from sheep and goats and all of them gave a characteristic common band at 280 bp. The histopathological examination revealed that the severity of both sheep and goats infection with M. ovipneumoniae is greater than those infected with M. agalactiae and/or M. arginini. Lesions were mainly found in the lungs in the form of fibrinous pneumonia with pleuritis. Hepatocellular necrosis, lymphoid depletion in the spleen and bronchial lymph nodes as well as degeneratic changes and necrosis of the tracheal and intestinal epithelium

Severe form of frontonasal dysplasia [FND] with bilateral anophthalmia: a case report with review of literature

Frontonasal Dysplasia [FND] is a rare disorder characterized by deformities of variable severity affecting the craniofacial region. FND with bilateral anophthalmia has not been reported yet in the literature. Maxillofacial surgery should be considered for all patients for whom improvement is possible. But in countries like Pakistan where there are considerable limitations in provision of social services, with economic and educational constraints, correction of such major defects remains a challenging task. In this report in an infant a very severe form of frontonasal dysplasia with bilateral anophthalmia with review of literature is presented

Congenital pyloric atresia [CPA]: report of two cases, with review of literature

We are reporting two neonates with isolated pyloric atresia, with an objective to emphasize the importance of considering this rare condition in the differential diagnosis of upper intestinal atresias. Both had non-bilious vomiting and epigastric fullness. X-ray showed a dilated gastric shadow with no evidence of gas in the rest of the abdomen. On exploration they had pyloric atresia which was corrected with gastroduodenostomy. Congenital pyloric atresia is a rare condition, which presents with features of gastric outlet obstruction. It can be diagnosed antenatally but the picture can mimic other conditions. Epidermolysis bullosa has a strong association with it and require skin biopsy for diagnosis