Recent isolation and identification of equine herpes viral abortion [EHV-1] in Egypt-2007

The present study was planned to isolate and identify the local Equine herpes viral abortion [EHV-1]. So, samples [liver, lung, spleen, placenta and serum] of 5 aborted mares and their foeti were collected from a private stud in Egypt, with a history of recurrent abortion during [2005, 2006]. Attempts for isolation of the causative agent were performed on chorioallantoic membrane of SPF enibryonated chicken eggs and tissue culture [VERO cells]. The highest titre was obtained from the liver of foeti which ranged from 8-9 log10 EID50 at 3[rd] egg passage and 7-8 logio TCID50 at 5[th] tissue culture passage. These results were confirmed by different serological tests [AGPT, VN test, ELISA and IF] by using reference rabbit anti-EHV-1 sera. In addition, antibodies against EHV-1 were detected in mares sera by AGPT and ELISA

Preliminary trials for the improvement of the locally produced inactivated RVF vaccine

After the last outbreak of RVF in Egypt during 1993, research works were directed towards the development of a much higher immunogenic and more potent inactivated RVF vaccine. The present work reports on the production of a much safer, more economic and potent inactivated vaccine as well as increasing its shelf live validity. The vaccine was safe when tested in suckling mice and potent when tested in adult mice and sheep. It had an ED[50] of 0.0005/ml. Yet, this vaccine has to be tried on a large scale to judge upon its validity for field use and/or replacing the present classical inactivated one