Extended spectrum beta- lactamase [EBSL] in E. coli isolated from a tertiary hospital in Enugu state, Nigeria

The frequency of extended spectrum beta - lactamase [ESBL] producing E. coil in a tertiary hospital in Enugu, Enugu State, Nigeria was studied. Clinical specimens were collected from patients attending the Medical Microbiology Laboratory unit of the University of Nigeria Teaching Hospital [UNTH], Enugu. Isolates of E. coil were obtained from various specimen types namely, urine [19]; blood [25], wound [52], and sputum [32]. Susceptibility studies were carried out using agar diffusion method by Kirby and Bauer, while ESBL detection on these isolates was carried out on Mueller Hinton agar using the double disc diffusion method. The frequency of ESBL producing E. coli among the clinical isolates was 11.4% [15] and ESBL producing organisms were isolated more frequently from blood [6] followed by wound [5], urine [3] and sputum [1]. Urgent measures to avert the further spread of ESBL producing organisms in Enugu, Enugu State, Nigeria is a compelling necessity

Antibiotic sensitivity pattern of staphylococcus aureus in Abakaliki, Nigeria

To investigate the sensitivity pattern of Staphylococcus aureus isolates obtained from clinical specimens including urine, wound, high vaginal swab and semen to commonly used antibiotics. The susceptibility patterns of these isolates were determined using the disc diffusion and agar well diffusion methods. Out of 174 samples, 51 [29.2%] yielded S. aureus with the highest isolation from semen [66.7%] and the least from urine [15.6%]. Following the disc diffusion, the highest sensitivity was recorded for Ofloxacin [65%] while the least was for Co-trimoxazole [6%]. Amoxicillin, Ampicillin, Tetracycline and Cloxacillin recorded 37%, 19%, 8% and 11% respectively. Also, following the agar well diffusion method, the highest sensitivity was recorded for Ofloxacin [65%] and the least for Co-trimoxazole [8%]. The other drugs recorded the following readings; Amoxycillin [37%], Ampicillin [21%], Tetracycline [23%] and Cloxacillin [13%]. The study recommends the use of Ofloxacin in the treatment of S. aureus infections in the study area. It also underscores the need for sensitivity testing before the administration of antibiotics for the treatment of Staphylococcal infections