Effects of Se nanoparticles supplementation on growth performance, hematological parameters and nutrient digestibility of Labeo rohita fingerling fed sunflower meal based diet

Abstract The aim of the present study is to assess the effects of selenium nanoparticles on the growth, hematology and nutrients digestibility of Labeorohita fingerlings. Fingerlings were fed with seven isocaloric sunflower meal-based diet supplemented with different concentrations of nanoparticles naming T1 to T7 (0, 0.5, 1, 1.5, 2, 2.5, and 3 mg/kg), with 5% wet body weight while chromic oxide was used as an indigestible marker. After experimentation for 90 days T3 treated group (1mg/kg -1Se-nano level) showed the best result in hematological parameters (WBCs 7.97 ×103mm-3, RBCs 2.98 ×106 mm-3 and Platelet count 67), nutrient digestibility (crude protein: 74%, ether extract: 76%, gross energy: 70%) and growth performance (weight gain 13.24 g, weight gain% 198, feed conversion ratio 1.5, survival rate 100%) as compared to the other treatment groups. Specific growth rates were found significantly higher in T5 than in other groups. The present study indicated positive effect of 1 mg/kg Se-nanoparticles on growth advancement, hematological parameters, and nutrients digestibility of L. rohita fingerlings.

Resumo O objetivo do presente estudo é avaliar os efeitos das nanopartículas de selênio no crescimento, hematologia e digestibilidade dos nutrientes de alevinos de Labeo rohita. Os alevinos foram alimentados com sete dietas isocalóricas à base de farinha de girassol suplementada com diferentes concentrações de nanopartículas, nomeando T1 a T7 (0, 0,5, 1, 1,5, 2, 2,5 e 3 mg / kg), com 5% do peso corporal úmido enquanto o óxido crômico foi usado como um marcador indigesto. Após a experimentação por 90 dias, o grupo tratado com T3 (nível 1mg / kg -1Se-nano) mostrou o melhor resultado em parâmetros hematológicos (WBCs 7,97 × 103mm-3, RBCs 2,98 × 106mm-3 e contagem de plaquetas 67), digestibilidade dos nutrientes (proteína bruta: 74%, extrato de éter: 76%, energia bruta: 70%) e desempenho de crescimento (ganho de peso 13,24 g, ganho de peso % 198, taxa de conversão alimentar 1,5, taxa de sobrevivência 100%) em comparação com os outros grupos de tratamento. As taxas de crescimento específicas foram encontradas significativamente mais altas em T5 do que em outros grupos. O presente estudo indicou efeito positivo de 1 mg / kg de nanopartículas de Se no avanço do crescimento, parâmetros hematológicos e digestibilidade de nutrientes de alevinos de L. rohita.

Effects of Se nanoparticles supplementation on growth performance, hematological parameters and nutrient digestibility of Labeo rohita fingerling fed sunflower meal based diet / Efeitos da suplementação de nanopartículas de Se no desempenho de crescimento, parâmetros hematológicos e digestibilidade de nutrientes da dieta baseada em farinha de girassol alimentada com alevinos de Labeo rohita

Abstract The aim of the present study is to assess the effects of selenium nanoparticles on the growth, hematology and nutrients digestibility of Labeorohita fingerlings. Fingerlings were fed with seven isocaloric sunflower meal-based diet supplemented with different concentrations of nanoparticles naming T1 to T7 (0, 0.5, 1, 1.5, 2, 2.5, and 3 mg/kg), with 5% wet body weight while chromic oxide was used as an indigestible marker. After experimentation for 90 days T3 treated group (1mg/kg -1Se-nano level) showed the best result in hematological parameters (WBC's 7.97 ×103mm-3, RBC's 2.98 ×106 mm-3 and Platelet count 67), nutrient digestibility (crude protein: 74%, ether extract: 76%, gross energy: 70%) and growth performance (weight gain 13.24 g, weight gain% 198, feed conversion ratio 1.5, survival rate 100%) as compared to the other treatment groups. Specific growth rates were found significantly higher in T5 than in other groups. The present study indicated positive effect of 1 mg/kg Se-nanoparticles on growth advancement, hematological parameters, and nutrients digestibility of L. rohita fingerlings.

Resumo O objetivo do presente estudo é avaliar os efeitos das nanopartículas de selênio no crescimento, hematologia e digestibilidade dos nutrientes de alevinos de Labeo rohita. Os alevinos foram alimentados com sete dietas isocalóricas à base de farinha de girassol suplementada com diferentes concentrações de nanopartículas, nomeando T1 a T7 (0, 0,5, 1, 1,5, 2, 2,5 e 3 mg / kg), com 5% do peso corporal úmido enquanto o óxido crômico foi usado como um marcador indigesto. Após a experimentação por 90 dias, o grupo tratado com T3 (nível 1mg / kg -1Se-nano) mostrou o melhor resultado em parâmetros hematológicos (WBC's 7,97 × 103mm-3, RBC's 2,98 × 106mm-3 e contagem de plaquetas 67), digestibilidade dos nutrientes (proteína bruta: 74%, extrato de éter: 76%, energia bruta: 70%) e desempenho de crescimento (ganho de peso 13,24 g, ganho de peso % 198, taxa de conversão alimentar 1,5, taxa de sobrevivência 100%) em comparação com os outros grupos de tratamento. As taxas de crescimento específicas foram encontradas significativamente mais altas em T5 do que em outros grupos. O presente estudo indicou efeito positivo de 1 mg / kg de nanopartículas de Se no avanço do crescimento, parâmetros hematológicos e digestibilidade de nutrientes de alevinos de L. rohita.

Missense mutations in the pancreatic beta-cell ATP-sensitive potassium channel Kir6.2: a case study of Pakistani patient of neonatal diabetes

This case study describes clinical and molecular genetic data of a 45 days old male patient of neonatal diabetes mellitus. PCR amplification followed by DNA sequencing revealed two point mutations at positions 67A>G and 1009G>A in KCNJ11 gene encoding Kir6.2 protein, a component of the beta-cell ATP-sensitive potassium [KATP] channel which is a key component involved in insulin secretion

Percutaneous radiologic gastrostomy: results and complications

Percutaneous endoscopic gastrostomy is usually difficult in patients with malignant involvement of oral cavity, pharynx and esophagus. Flouroscopic guided insertion of Gastrostomy catheter with Gastropexy have gained acceptance because it is easy and less time consuming as well as less invasive as compare to surgical procedure[5]. This study was done to evaluate the safety and efficacy of percutaneous placement of gastrostomy with gastropexy using imaging guidance in patients with oropharyngeal and esophageal cancers. Over five years, 105 patients were referred to our department for percutaneous radiologic gastrostomy. In five patients the procedure was not performed because of overlying viscera and high position of stomach. We performed 100 gastrostomies with gastropexy procedures using seldinger technique. Success rate for percutaneous radiologic gastrostomy was 100%. No major complication had occurred. There were 11 minor complications occurred including 4 stomal infection, 3 catheter obstruction, one peritonism and three were extensive pneumoperitoneum. Stomal infection and catheter obstruction were not related to procedure. So, our true minor complications were only 4 [4%] which is comparable to literature. Percutaneous radiologic gastrostomy is an effective and safe procedure for enteric access of nutrition in patients with oral, pharyngeal and esophageal cancer where percutaneous endoscopic gastrostomy is difficult

Radiological evaluation of obstructive jaundice

A study involving 125 patients of obstructive jaundice was conducted in Combined Military Hospital and Military Hospital Rawalpindi. The study group predominantly consisted of female patients between 40 and 60 years. Ultrasonography and endoscopic retrograde cholangiopancreatography [ERCP] were the commonest radiological investigations used. Ultrasonography [USG] was performed in 100% patients. It successfully differentiated obstructive from non-obstructive jaundice. ERCP was perfomed in 100 patients [80%]- It was helpful in permitting direct, non-surgical imaging of pancreatic and bilary ducts. It proved to be useful diagnostic tool as it provided gastroduodenoscopy, cholangiography and pancreatography with a single examination. Percutaneous transhepatic cholangiography [PTC] was helpful in assessing the presence, site and cause of obstruction

Diagnostic accuracy of ultrasonography and percutaneous transhepatic cholangiography in pereoperative evaluation of obstructive jaundice

A study was conducted in Radiology Department, Combined Military Hospital, Rawalpindi to determine the combined efficacy of Ultrasonography [USG] and Percutaneous Transhepatic Cholangiography [PTC] in the preoperative evaluation of obstructive jaundice. After initial screening by means of Ultrasonography, 22 patients were selected for PTC. Of them 14 underwent surgery, providing an opportunity for assessing the separate and combined accuracy of both the modalities. Both were equally accurate detecting stones in the common bile duct [100%]. In assessing stones of the gallbladder, USG [100%] was found to be superior to PTC [0%]. Similarly, USG [87.5%], outperformed PTC [37.5%] in identifying enlargements of the gallbladder. PTC [87.5%] however, surpassed USG [28.5%] in detecting extrinsic compression of the common bile duct [CBD] by nepotistic pancreatic enlargements. Combination of these two modalities resulted in a much improved diagnostic yield and proved to be an economical and safe yet formidable means of preoperative assessment of obstructive jaundice without the need for the more expensive CT scan, or the less available endoscopic retrograde cholangiopancreatography [ERCP]

Radiological evaluation of obstructive uropathy in children

Early detection of obstructive uropathy in children is of crucial importance as it leads to prevention of a number of complications and impending renal failure. A series comprising of 51 patients from both sexes up to the age of 12 years was studied. The imaging modalities used were plain films of abdomen [KUB], ultrasonography [USG], intravenous urography [IVU], micturating cystourethrography [MCUG] and ascending [retrograde] pyelography. The commonest cause of obstruction seen was due to calculi in the renal tract. Ultrasonography proved to be highly sensitive in picking up early cases of obstruction. Male children were affected more often than female children. Obstruction in urinary passage at all levels was seen, extending from kidneys to the urethra. It was also seen that obstruction was unilateral, bilateral, acute, chronic, complete, incomplete, and painful or silent. In view of our study, routine screening ultrasound examination of every child is advised to detect silent cases of unilateral obstruction

role of barium enema in the evaluation of morphologic and histopathologic characteristics of colorectal tumours

During a 9-months period [Sep 1991-Jun 1992] clinical, radiographic, endoscopic, surgical and histopathologic findings of 25 patients with colorectal tumours were reviewed retrospectively. To assess the current status of barium enema examination as the initial diagnostic procedure for the detection of suspected colorectal tumours and to determine the relationship between their morphologic and histopathologic characteristics. Six patients [24%] had lesions proximal to the splenic flexure and 19 [76%] patients had more distal lesions predominantly [68%] in the rectosigmoid region. Large [>4 cm] intraluminal fungating cancers [34.50% [9 of 25 patients]] and long [> 4 cm] rigid neoplastic strictures [30.75% [8 of 29 patients]] were among the commonest morphologic forms of colorectal tumours. Four [[out of 25] [15.40%]] patients had typical annular carcinoma; two [[out of 25] [7.70%]] patients had small [2-4 cm] polypoidal growths and one [[out of 25] [3.85%]] had a flat marginal lesion. One patient had double lesions so two polyps [7.70%] were also detected. The minimum size of the lesion noted was 1.8 cm and maximum was 16.2 cm. Histopathologic reports revealed 2 adenomatous polyps; 2 metastatic cancers; on squamous sell carcinoma and 20 cases of primary colorectal adenocarcinomas; 2 [10%] were well-differentiated; 9 [45%] moderately differentiated; and 9 [45%] poorly differentiated. Three patients had Dukes stage A lesions; 10 had Dukes stage B lesions; 8 had Dukes stage C lesions; and 4 had Dukes stage D lesions. Long strictures and annular carcinomas were more likely to extend to the stroma and spread to the lymph nodes. The results showed that double contrast barium enema examination is an easy and valuable initial diagnostic procedure for the detection of suspected colorectal tumours and is also significantly helpful in predicting their morphologic and histopathologic characteristics