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1.
Braz. j. microbiol ; 46(1): 29-39, 05/2015. tab, graf
Artículo en Inglés | LILACS | ID: lil-748236

RESUMEN

Awareness on antioxidants and its significance in human healthcare has increased many folds in recent time. Increased demand requisite on welcoming newer and alternative resources for natural antioxidants. Seaweed associated pigmented bacteria screened for its antioxidant potentials reveals 55.5% of the organisms were able to synthesize antioxidant compounds. DPPH assay showed 20% of the organisms to reach a antioxidant zone of 1 cm and 8.3% of the strains more than 3 cm. Pseudomonas koreensis (JX915782) a Sargassum associated yellowish brown pigmented bacteria have better activity than known commercial antioxidant butylated hydroxytoluene (BHT) against DPPH scavenging. Serratia rubidaea (JX915783), an associate of Ulva sp. and Pseudomonas argentinensis (JX915781) an epiphyte of Chaetomorpha media, were also contributed significantly towards ABTS (7.2% ± 0.03 to 15.2 ± 0.09%; 1.8% ± 0.01 to 15.7 ± 0.22%) and FRAP (1.81 ± 0.01 to 9.35 ± 0.98; 7.97 ± 0.12 to 18.70 ± 1.84 μg/mL of AsA Eq.) respectively. 16S rRNA gene sequence analysis revealed bacteria that have higher antioxidant activity belongs to a bacterial class Gammaproteobacteria. Statistical analysis of phenolic contents in relation with other parameters like DPPH, ABTS, reducing power and FRAP are well correlated (p < 0.05). Results obtained from the current study inferred that the seaweed associated pigmented bacteria have enormous potential on antioxidant compounds and need to be extracted in a larger way for clinical applications.


Asunto(s)
Antioxidantes/metabolismo , Organismos Acuáticos/clasificación , Organismos Acuáticos/metabolismo , Gammaproteobacteria/clasificación , Gammaproteobacteria/metabolismo , Algas Marinas/microbiología , Organismos Acuáticos/genética , Organismos Acuáticos/aislamiento & purificación , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Gammaproteobacteria/genética , Gammaproteobacteria/aislamiento & purificación , Datos de Secuencia Molecular , Pigmentos Biológicos/metabolismo , /genética , Análisis de Secuencia de ADN
2.
J Environ Biol ; 2011 Mar; 32(2): 213-219
Artículo en Inglés | IMSEAR | ID: sea-146568

RESUMEN

Estuaries and tidal creeks, harboring mangroves particularly, face tremendous anthropogenic pressures. Expansion of mega cities and the thermal power plants are generally proposed in the vicinity of estuaries and creek, due to the feasibility of intake and discharge of water for cooling. Discharges from such developments remain constant threat of increasing thermal pollution and affecting the quality of environment. The baseline information on prevailing quality of aquatic environment comes handy for understanding alterations due to such activities. Principle component analysis (PCA) revealed that temperature, pH, salinity, suspended solids, DO, BOD and phaeophytins are major parameters influencing the creek system. Heated effluents may have direct and adverse impacts on these parameters, altering biotic constituents. Hence, periodic and detailed observations are necessary to estimate exact response of biotic communities to changing environment. The present paper is based on case study, projecting a power plant in the vicinity of major mangrove habitats of Dharamtar creek.

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