Study of toxicity and haemagglutinating activitiy of a purified intracellular toxin and extracellular crude toxin isolated from pseudomonas aeruginosa

The action of purified intracellular toxin [PIT] from Pseudomonas aeruginosa was examined in brine shrimp lethality bioassay. The LC50 of the PIT was calculated to be 25 microg/ml. The PIT agglutinated both albino rat and rabbit erythrocytes more potently than did extracellular crude toxin [ECT]. Galactose and Dmannose, however inhibited the agglutination property of PIT and ECT respectively. Intradermal injection of PIT caused changes on the tissues of rabbit skin at a lower dose than that of ECT