Discrimination of dominant helicobacter pylori strains isolated from patients with different gastroduodenal pathologies by protein profiling

It is not clear what factors determine divergent outcomes of infections caused by H. pylori. In the present study, the protein profiles of different strains of H. pylori, isolated from three groups of patients with ulcerative disease, non-ulcerative gastritis and cancer disease, were analyzed using 1D-SDS-PAGE. The patterns of different H. pylori strains were highly divergent. About 30.76% [7 bands] of the 26 observed protein bands were common in all strains isolated from 3 groups of the patients. While the similarity for the strains inside each group were 75% [15 from 20], 76.47% [13 from 17] and 78.57% [11 from 14] for cancerous, ulcerative and nonulcerative group, respectively. Some of the observed bands were significantly specific for each group. Therefore, we speculated that some H. pylori strains might be more associated with a specific disease than others, giving the clustering of some, but not all, strains within each disease group. In conclusion, this study showed that protein profile can be a characteristic in discrimination of dominant strains in different gastric clinical status. Specific and dominant proteins of different strains isolated from three groups of patients under study were candidates for further exploration for laboratory tests, which analyze disease-specific H. pylori strains, and for diagnosis of the different diseases and outcomes associated with this widespread bacterium

Immunodominant antigens of helicobacter pylori strains isolated from patients with different gastroduodenal diseases

To detect the immunogenic proteins in Helicobacter pylori [H. pylori] strains isolated from patients with different gastric diseases. We performed this study in the Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, during July 2003 to September 2004. Total proteins of H. pylori strains isolated from the gastric biopsies of 3 groups of patients were separated by 1D-SDS-PAGE and then blotted with the sera of their respective hosts. In SDS-PAGE the members of each group showed high correlation according to similarity in their patterns, resulting in considering them in the same cluster. The patterns of immunoblots differed from that of Coomassie Brilliant Blue stained gels. The blotting method did not recognize some of the protein bands in the SDS-PAGE. Only the bands of 106 and 45 kDa from H. pylori strains isolated from patients with gastric cancer were significantly [p<0.05] recognized specifically with the sera of their respective patients, and the band of 13 kDa was recognized specifically [p<0.05] with the sera of nonulceric patients. With the exception of these bands, in the patterns of blotting of the sera from all patients no significant differences were observed. By using 1D blotting methods we could find 2 antigenic protein bands [106 and 45 kDa] for H. pylori strains isolated from cancerous patients, and one [13 kDa] for the strains isolated from nonulceric patients, which were specifically recognized with their respective host