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Background and Aim: Recently, several studies have indicated that the central nervous system has the capacity for endogenous repair. But, the proliferation capacity of endogenous neural stem cells [NSCs] isn't sufficient for the treatment of neurodegenerative diseases. So, it sounds that stimulation of endogenous NSC proliferation is essential for neuroregeneration. The aim of this study was to examine the effects of Sambucus ebulus extract on the proliferation of neonatal rat hippocampus-derived neural stem cells [NSCs] under oxidative stress condition induced by H2O2
Material andmethods: The NSCs were isolated from neonatal rat hippocampus. To confirm neural characteristics of neural stem cells, the expression of neural-specific marker, Nestin was investigated by immunocytochemistry technique. 5×104 cells were cultured in every well of a 96 well plate and H2O2 was added to induce oxidative stress condition. Then NSCs were exposed to 50 microgram Sambucus ebulus extract for 24 hours, at various concentrations [25, 50, 100, 200, 400 and 500 microgram/ml]. The cell proliferation rate was assessed by MTT colorimetry assay before and after treatment with the extract
Results: Immunofluorescent studies showed that neural stem cells expressed specific neural marker; Nestin. The proliferation rate of NSCs increased in the treated groups in comparison to that in the control group. The highest rate of survival was observed when Sambucus ebulus was used at the concentration of 500 microgram/ml. [P<0.05]
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OBJECTIVE@#To evaluate the effect of doxorubicin and its pegylated liposomal formulation (Doxil, Caelyx) on in vitro susceptibility of promastigote and amastigote stages of Leishmania major.@*METHODS@#Throughout in vitro assays the IC was calculated in the promastigotes and amastigotes forms in J774 macrophage cell line. Also as cytotoxicity in J774 cell line macrophages.@*RESULTS@#Doxorubicin and Doxil showed the same activity against promastigote form with IC values of 10.49 μg/mL and 9.63 μg/mL, respectively. Similarly, the amastigote stage was susceptible at concentration of at least 1 μg/mL when compared to positive control (P < 0.0001). Also, cytotoxicity assay against macrophage revealed no toxicity on the host cells at IC concentrations.@*CONCLUSIONS@#Our findings demonstrated the efficacy of both doxorubicin and its pegylated liposomal formulation on L. major at low concentrations. Further researches are needed for evaluating the safety of drugs in animal model particularly as topical formulation.
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Objective To evaluate the effect of doxorubicin and its pegylated liposomal formulation (Doxil, Caelyx) on in vitro susceptibility of promastigote and amastigote stages of Leishmania major. Methods Throughout in vitro assays the IC