RESUMEN
Cardiospermum halicacabum Linn. (Sapindaceae) is a climber, commonly found throughout India, used as vegetable as well as traditional medicine for rheumatism, lumbago and nervous diseases. In the current study, we investigated the antioxidant potency of successive extracts of C. halicacabum seeds employing various established in vitro systems, such as the lipid peroxidation, nitric oxide radical scavenging and reduced glutathione assay. Furthermore, all the extracts exhibited concentration dependent inhibition in all the models at tested doses 25-1000 μg/ ml. The data obtained in the in-vitro models clearly establish the antioxidant potency of C. halicacabum seed extract.
RESUMEN
Breast cancer is the leading cause of cancer associated death among women worldwide. Current cancer treatments include chemotherapy, radiotherapy, and surgery. However, since these conventional methods often have undesirable side effects,new focus towards the use of plant extract to treating cancer with eliminating the side effects. The objective of present study is to asses the anticancer effect of leaves of Alstonia scholaris, using the cytosolic marker enzymes like Aspartate transaminase (AST),Acid phosphatase(ACP) , Alkaline phosphatase(ALP), Lactate dehydrogenase (LDH), Gamma - glutamyl transferase(γ– GT), and 5'-nuclcotidase(5'-NT) in vitro over breast cancer tissue. These are key enzymes in the metabolic pathways and these are the target for the drugs used in chemotherapy. An elevated level of enzyme concentration signals the presence of malignancy .The effect of leaves of Alstonia scholaris is also assessed by studying the effect of non-enzymatic antioxidants like vitamin A, E, C in vitro over breast cancer tissue. The present study revealed the leaves of methanolic extracts of Alstonia scholaris on cancer cells/tumor cells in vitro has been justified by its cytotoxic effect and anti proliferative effect.