Localization and mRNA Expression of Angiogenin in Bovine Ovary / 대한해부학회지

Ovary is one of the organs in which angiogenesis occurs during ovarian cycle. Angiogenesis is associated with angiogenic factor like acidic fibroblast growth factor, basic fibroblast growth factor and transformation growth factor. Therefore, we performed this study to identify the distribution and mRNA expression of angiogenin, new potential angiogenic factor, in ovary of Korean native cattle by immunohistochemistry and in situ hybridization. Angiogenin immunoreactivity and mRNA expression were observed in endothelial cells, fibroblast and vascular smooth muscle cells. However, we could not observed angiogenin immunoreactivity and mRNA expression in primordial ovarian follicle. In follicular epithelial cells of primary ovarian follicle, weak angiogenin immunoreactivity and mRNA expression were observed. Follicular epithelial cells, theca interna and externa in secondary ovarian follicles, showed angiogenin immunoreactivity, while follicular epithelial cells did the weak mRNA expression. Angiogenin immunoreactivity and mRNA expression were observed in follicular epithlial cells, theca interna and oocyte in tertiary ovarian follicle. The corpus luteum showed strong immunoreactivity and mRNA expression but atretic follicle weak. However, these angiogenin immunoreactivity and mRNA expression became to be weaker during regression. These results suggest that angiogenin may play a role as not only an angiogenic factor but a growth factor in ovary.

Double Immunohistochemical Studies on Distribution and Coexistence with Putative Neurotransmitters and Calretinin in Trigeminal Ganglion of Korean Native Goat / 대한해부학회지

The present study was undertaken to investigate the morphological characteristics of trigeminal ganglion in Korean native goat (Capra hircus) by immunohistochemical methods. The calcitonin gene-related peptide (CGRP), substance P (SP), galanin (GAL) and calretinin (CR) immunoreactivities by immunohistochemical method were observed in the neurons of trigeminal ganglion with populations of 43.75%, 26.01%, 4.98%, and 14.33%, respectively. In double immunohistochemical study, CGRP immunoreactivity was proven to be present in SP (93.93%) and GAL (100%) immunoreactive neurons. SP immunoreactivity was observed in CGRP (36.12%) and GAL (100%) immunore-active neurons. GAL immunoreactivity was colocalized with 8.14% of CGRP and 15.47% of SP immunoreactive neurons. However, CR immunoreactivity was not observed in CGRP, SP and GAL immunoreactive neurons. These findings exhibit that Korean native goat differs from other mammalian species in the distribution and localization of neurochemical substances in trigeminal ganglion, and suggest that these differences may be related with neuroanatomical characteristics.