Bletilla striata planting area in Ningshan county extraction based on multi-temporal remote sensing images / 中国中药杂志

Traditional Chinese medicine is planted in mountainous areas with suitable natural conditions. The planting area is complex in terrain,and the planting plots are mostly irregularly shaped. It is difficult to accurately calculate the planting area by traditional survey methods. The method of extracting Chinese herbal medicine planting area combined with remote sensing and GIS technology is of great significance for the rational development and utilization of traditional Chinese medicine resources. Taking Bletilla striata planting in Ningshan county of Shaanxi province as an example,the extraction method of planting area of traditional Chinese medicine in county was studied. High-resolution ZY-3 and GF-1 multi-spectral multi-temporal remote sensing images were used as data sources. Through field sampling,samples such as B. striata,cultivated land,forest land,water body,artificial surface,alpine meadow,etc. are collected. The spectral features,texture features and shape features of remotely identifiable objects in different planting areas and cultivated land,vegetable sheds were analyzed,confusing ground objects were eliminated and interpretation marks were establish. The method of visual interpretation is used to realize the extraction of B. striata planting areas,and the B. striata planting area are calculated by combining GIS technology. The results showed that the method of visual interpretation,using high-resolution ZY-3 and GF-1 multi-spectral multi-temporal remote sensing image data extracted the planting area of 403.05 mu. It can effectively extract the B. striata planting area in research region.

Determination of quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li. by HPLC / 中国药学杂志

OBJECTIVE: To establish a method to determine quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li. METHODS: Diamonsil C18 column was used. Methanol-0.4% glacial acetic acid solution was used as the mobile phaseat a flow rate of 1 mL · min-1. The column temperature was 30°C. The detection wavelength was 290 nm. RESULTS: The liner ranges of quercetin, podophyllotoxin and kaempferol were 0.023-0.287 μg (r=0.999 9), 0.208-2.6 μg (r=0.999 9), and 0.036 1-0.451 μg (r=0.999 9), respectively. The average recoveries were 99.64% (RSD=0.83%), 100.04% (RSD=0.61%), and 99.88% (RSD=1.19%), respectively. CONCLUSION: The method is sensitive, accurate and reproducible. It can be used for the determination of quercetin, podophyllotoxin and kaempferol in Diphylleia sinensis Li.