Identification of active components of Dendrobium inhibit growth of gastric cancer cells based on component-activity relationship / 中国中药杂志

Three cancer cell lines including gastric cancer SGC-7901, HGC-27, and MGC-803 cells were employed to evaluate the bioactivity of seven Dendrobium species. Simultaneously, these Dendrobium species were assessed with UPLC-Q-TOF-MS, and 504 common peaks were found. Based on the hypothesis that biological effects varied with differences in components, multivariate relevance analysis for chemical component-activity relationship of Dendrobium, including grey relation(GRA) and partial least squares(PLS) analysis were performed to evaluate the contribution of each identified component. The target peaks were identified by standards toge-ther with databases of Dendrobium, Nature Chemistry, MassBank, etc. Finally, four active components, including 3,5,9-trihydroxy-23-methylergosta-7,22-dien-6-one, diacylglycerol(14∶1/22∶6/0∶0), pipercitine, and 22-tricosenoic acid, might have negative effect on the growth of gastric cancer cells.

Far Upstream Element-Binding Protein 1 (Fubp1) Expression in Tongue Carcinoma and Its Relationship with the Clinicopathological Parameters and Prognosis / 中山大学学报(医学科学版)

[Objective] To investigate the far-upstream element (FUSE) binding protein 1 (FUBP1) expression in tongue squamous cell carcinoma (TSCC) and its adjacent normal tissues,to explore the role of FUBP1 in the initiation and development of TSCC and its clinical significance.[Methods] FUBP1 expression in TSCC tissues and its adjacent normal tissues was detected by immunohistochemistry,qRT-PCR and Western blot respectively.Statistical analysis was used to analyze the relationship between FUBP1 expression and clinical pathological parameters and prognosis.[Results] FUBP1 expression in TSCC tissues was higher than that in the corresponding adjacent normal tissues (P＜0.05);FUBP1 expression in TSCC tissues was higher in patients of advanced stage and with cervical lymph node metastasis (P＜0.001);The 5-year survival rate of FUBP1 over expression group was lower than that of FUBP1 low expression group (P=0.035);FUBP1 may be an independent predictor in patients with TSCC.[Conclusion] The high expression of FUBP1 in TSCC tissues is associated with poor prognosis in patients with TSCC,therefore,high expression of FUBP1 may promote the formation and development of TSCC,and become a index for prognosis in TSCC patients.