RESUMEN
<p><b>OBJECTIVE</b>In order to investigate the positive rate of streptococcus suis type 2 and the genes of their suilysin (sly), extracellular protein (epf) and muramidasa-released protein ( mrp) and to understand the antibiotic susceptibility of S. suis type 2.</p><p><b>METHODS</b>S. suis type 2, isolated from slaughtered healthy pig's tonsil in 10 county area of Guangxi, were identified by Multiplex PCR, and the genes of their sly, epf, mrp and the antimicrobial sensitivity analysis were performed.</p><p><b>RESULTS</b>1105 strains of Streptococcus including 667 strains of S. suis and 33 strains of S. suis type 2 were detected from 1179 samples. In these S. suis type 2 strains, there were 22 strains of sly + mrp + epf+ type,1 strain of sly + mrp + epf - type, 2 strains of sly - mrp + epf + type, 7 strains of sly - mrp + epf - type and 1 strain of sly - mrp - epf- type. When these strains were subjected to be tested with penicillin, eritrocina, vacocin, gentamycin, specti-nomysin, enraxacin, ciprofloxaxin, cephalothin VI, sulfadiazine sodium, cyantin, mycifradin, amikacin and achromcin, some were found to be resistant to but most strains were susceptible to cephalothin VI, penicillin and enraxacin. There were 31, 29 and 27 strains over medium sensitivity, respectively, but 28 and 27 resistant strains to amikacin and achromcin were found.</p><p><b>CONCLUSION</b>The positive rate of S. suis type 2 in clinical healthy pigs was low (2.8%) and did not show obvious difference between the counties with or without a history of S. suis infection. All the isolated strains were susceptible to cephalothin VI, but most strains were virulent.</p>
Asunto(s)
Animales , Antibacterianos , Farmacología , Antígenos Bacterianos , Genética , Proteínas Bacterianas , Genética , Farmacorresistencia Bacteriana , Genética , Proteínas Hemolisinas , Genética , Pruebas de Sensibilidad Microbiana , Epidemiología Molecular , Métodos , Reacción en Cadena de la Polimerasa , Infecciones Estreptocócicas , Epidemiología , Genética , Microbiología , Streptococcus suis , Genética , Virulencia , Porcinos , Enfermedades de los Porcinos , Epidemiología , Genética , MicrobiologíaRESUMEN
<p><b>OBJECTIVE</b>To observe the effect of Epimedium brevicornum, E. sagittatum, E. koreanum, E, wushanense and E. elongntum on the growth of cartilage and proliferation of cartilage cell in vitro.</p><p><b>METHOD</b>According to the paired design method, either femur of each chichen embryo was put into the control tube with the medium containing no Epimedium injection, and the other was put into the treated tube with the medium containing 3% Epimedium injection. The length and weight of cultured cartilage and proliferation of cartilage cell (MTY method) were used as the indices to observe the cartilage biological activities of the five species of Epimedium in culture.</p><p><b>RESULT</b>The results showed that the indices of length, weight and MTT in the E. brevicornum and E. sagittatum group were significantly higher than those in the contral group, but the above indices in groups E. koreanum, E. wushanens and E. elongntum were similar to those of the control with no statistical difference.</p><p><b>CONCLUSION</b>E. brevicornum and E. sagittatum can improve the growth of cartilage and proliferation of cartilage cell in vitro, and other three Epimedium have not the same effect in this test.</p>