Preparation and identification of a recombinant hepatitis C virus (HCV) based on sindbis virus vector / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To construct the recombinant virus-like particles containing HCV envelope glycoprotein E1E2 based on sindbis virus vector.</p><p><b>METHODS</b>The gene encoding HCV envelope glycoprotein E1E2 was cloned into sindbis virus vector to construct recombinant plasmids pBR-XJE1E2 and pVA-XJE1E2, and transfect them into BHK-21 cells to obtain recombinant virus-like particles. The expression of E1 and E2 protein were verified by Western Blot and indirect immunofluorescent assay (IFA).</p><p><b>RESULTS</b>The results of restriction enzyme digestion, PCR and sequencing analysis showed that the recombinant plasmids were constructed successfully. And the results of RT-PCR, Western blotting and IFA detection showed that the transfect cells could package HCV-like particles of expressing structural proteins E1E2.</p><p><b>CONCLUSION</b>The recombinant expression plasmids pBR-XJE1E2 and pVA-XJE1E2 based on sindbis virus vector could package HCV-like particles in eukaryotic cell, which provides a foundation for further study of its in vivo animal immune response.</p>

The diagnostic value of axial loading imaging of the lumbar spine during CT and MR examination in patients with degeneration disorders / 中华放射学杂志

15mm~2)of dural sac cross-sectional area to values smaller than 75 mm~2 was found during examination in axial loading,or if a suspected disc herniation,narrow lateral recess,narrow intervertebral foramen,or intraspinal synovial cyst changed to being obvious at the axial loading examination,they were regarded as additional information.Results After axial loading CT examination,AVI was found in 16 of 40 patients.A significant decrease of dural sac area was found in 13 patients.Intervertebral disc herniation was more severe in 7 patients,lateral recess or interverbral foramen narrowed in 4 patients,no intraspinal synovial cyst was found.After axial loading MRI examination,AVI was found in 19 of 60 patients.A significant decrease of dural sac area was found in 13 patients.Intervertebral disc herniation became severe in 10 patients,lateral recess or interverbral foramen narrowed in 8 patients,no intraspinal synovial cyst was found.AVI was found in 32 of 79(40.5%)patients with sciatica and 2 of 20(10.0%)patients with low back pain(?~2=7.45 P

The measurements of multiple slices CT on the narrow pharyngeal cavity in obstructive sleep apneahypopnea syndrome / 中华放射学杂志

Objective To define the characteristic values of the pharyngeal cavity by comparing the values of measures and ratios between the patients with obstructive sheep apnea-hypopnea syndrome(OSAS) and controls.Methods Sixty-eight OSAS patients who were diagnosed using polysomnography,and 56 healthy people were scanned by MSCT(multiple slices computed tomography)in the awake state.Then, the values were measured on the reformated images and were compared between two groups.Results Of the 63 values,49 values were different between the two groups,and among those,4 values were entered into the Discriminant Functions.These were the left-right diameter(LR)[OSAS:LR=(9.4?3.7)mm,controls: LR=(20.1?5.0)mm,t=-13.820,P=0.000)and the cross-sectional area(XSA)[OSAS:XSA= (54?27)mm~2,controls:XSA=(164?77)mm~2,t=-10.944,P=0.000]of RP,the heights of tongue [OSAS:(75.4?8.3)mm,controls:(58.4?9.8)mm,t=10.476,P=0.000],the lengths of uvula/the lengths of airway(OSAS:0.139?0.039,controls:0.154?0.048,t=-1.983,P=0.050).Conclusions Many measures and ratios were different between the two groups,but there were overlaps between them. Measures using the Discriminant Functions can help the diagnosis of OSAS.