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Journal of Experimental Hematology ; (6): 1455-1458, 2015.
Artículo en Chino | WPRIM | ID: wpr-274017

RESUMEN

<p><b>OBJECTIVE</b>To identify a novel HLA allele DRB1 * 16:36 from a Uygur woman.</p><p><b>METHODS</b>PCR-SBT technology was applied to the extracted DNA for genotyping, and a possible new gene was sequenced by using sequence specific primers and single stranded SBT. This novel allele was compared with known most homologous gene sequences and their difference was analyzed.</p><p><b>RESULTS</b>This novel allele was different from HLA alle DRB1 * 16:23, and had highest similarity in 2 nucleotides at position 227 A→T and 236 T→C in exon 2, resulting in 3 amino acid changes from Tyr to Phe at codon 47 and Val to Ala at codon 50. The sequence of this novel allele had been submitted to GenBank.</p><p><b>CONCLUSION</b>This HLA allele DRB1 * 16:23 has been confirmed to be a novel allele, and has been officially named DRB1 * 16:36 by the World Health Organization (WHO) Nomenclature Committee in May 2015.</p>


Asunto(s)
Femenino , Humanos , Alelos , Secuencia de Bases , Cartilla de ADN , Exones , Genotipo , Cadenas HLA-DRB1 , Genética , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN
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