RESUMEN
OBJECTIVE A RP-HPLC method was establised for the determination of paeoniflorin and geniposide in Zhonggan oral liquid.METHODS The c18 column was used.The mobile phese was consisted of CH3CN-0.1% H3PO4(15∶85).The detection wavelength was at 230 nm.RESULTS The average recoveries were 98.78%(RSD=2.43%,n=5)for paeoniflorin and 99.60%(RSD=1.88%,n=5)for geniposide,respectively.CONCLUSION The method was simple,rapid and accurate.
RESUMEN
Both levels of serum leptin and BMI were significantly higher in T2DM patients with severe NAFL (n=61) than those in T2DM with mild NAFL (n=24), T2DM without NAFL (n=45) and in normal control (n=30).
RESUMEN
Objective: To determine the contents of paeoniflorin and glycyrrhizic acid in Gianggan Capsules by RP HPLC. Methods: The Chromatographic method was carried out on Kromasil C 18 column using acetonitrile phosphate buffer solution (pH=5) (20∶80) as a mobile phase. The detection wavelength was set at 230nm and 250nm. The flow rate was 1ml/min. The column temperature was 40℃. Results: The paeoniflorin and glycyrrhizic acid calibration curves were linear at the ranges of 0.48～4.80?g and 1.08～10.81?g(r=0.9999). The average recoveries were 100.5% with RSD 1.2% for paeoniflorin and 98.9% with RSD 0.9% for glycyrrhizic acid. Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Gianggan Capsules.