Determination of sinomenine and tanshinnone Ⅱ_A of Shuanghuoqufengzhentong Wine by HPLC / 中成药

AIM: A determination was created of sinomenine and tanshinnone Ⅱ A in Shuanghuoqufengzhentong Wine (Caulis Sinomenii, Radix Salviae Miltiorrhizae, etc.) at the same time by HPLC. METHODS: The method contained a Waters ? PORASIL TM P/N27477 column with methanol water ethylene diamine (90∶10∶0.125) as mobile phase, UV detection wavelength was set up at 262nm. RESULTS: The average recovery of sinomenine was 97.9%, the RSD was 2.83%. The average recovery of Tanshinnone Ⅱ A was 99.0%, The RSD was 1.96%. CONCLUSION: The method is rapid, accurate, sensitive. This study provides a testing method for the quality control of similar products.