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AIM:To explore the molecular mechanism through which curcumin reverses hepatocyte growth factor (HGF)-induced resistance to gefitinib in lung cancer cells.METHODS:The methods of MTT assay, wound healing assay and Western blot were used to observe the effects of HGF, curcumin and gefitinib on the migration, drug susceptibility, epithelial-mesenchymal transition, and related signaling pathways in the PC9 lung cancer cells.RESULTS:HGF reduced susceptibility of the PC9 cells to gefitinib, and curcumin significantly reversed HGF-induced resistance to gefitinib.HGF induced migration and epihelial-mesenchymal transition, and promoted c-Met/AKT/mTOR pathway activation in the PC9 cells.Gefitinib alone did not prevent the above activities.However, combined with curcumin, gefitinib prevented the above activities.CONCLUSION:Curcumin reverses HGF-induced resistance of the PC9 cells to gefitinib by preventing epithelial-mesenchymal transition and inhibiting c-Met/AKT/mTOR activation.
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Objegtive To study the feasibility and clinical effects of enteral nutrition by needle catheter jejunostomy(NCJ)tube in the postoperative adjuvant chemotherapy for patients of staged gastric cancer.Methods Eighty-two patients with staged gastric cancer underwent radical gastrectomy and going to receive chemotherapy were randomly divided into group A(41 cases)and group B(41 cases).All of the patients had been NCJ.Group A received enteral nutrition through the tube during chemotherapy,and group B had been given general diet.A series of parameters were measured post-chemotherapy.And the gastrointestinal complications were carefully observed.Results In post-chemotherapy,the level of hemoglobin,albumin,prealbumin,interleukin-2,natural killer cell activities and CD3+,CDd4+,CD4/CD8 in group A[(106.9±12.0)g/L,(26.2±1.4)g/L,(202.9±32.2)mg/L,(11.9±2.1)μg/L,(21.3±5.2)%,(62.9±3.3)%,(26.1±4.7)%,1.1±0.2]were significantly higher than those in group B(P＜0.05 or＜0.01).The incidences of vomiting in group A(4.9%,2/41)was significantly lower than that in group B (26.8%,11/41)(P＜0.05).The average intake in group A[(1312±114)ml]was significantly more than that in group B[(76.5±186)ml](P＜0.05).No severe enteral nutrition related complications occurred in group A.Conclusions It is safe and feasible to enteral nutrition supported by NCJ tube in chemotherapy for patients of staged gastric cancer.It can improve the nutrition status and immune function in the given patients.
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Objective To study the dissection technique of total mesoreetal excision(TME).Method One hundred and twelve eases of rectal cancer underwent TME from January 2000 to August 2008 were retrospectively analyzed.Results All the procedures were conducted sueeessfully,average blood loss was(45±32)ml during the dissection of rectal mesentery,the hypogastrie nerves were preserved in 96 eases,urinary retention occurred in 6 eases and recovered 1 month later.Anastomotie leakage occurred in 1 ease and healed after transverse colostomy.Wound infection in 6 eases and healed by dress changing.There was no ureter injury.There were 14 eases of death,6 eases of recurrence and 15 eases of distal metastasis according to(3.7±1.1)years follow-up in 112 eases.The survival rate of 1,3,5 years was 92.0%(103/112),84.8%(95/112),66.1%(74/112)respectively.Conclusion Anatomical dissection adequate exposure and hemostasis are critical factors for TME.