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Objective To investigate the expression and biological function of miRNA-449 a in lung cancer . Methods A case-control study was conducted in 58 patients diagnosed with lung cancer ( carcinoma and adeno-carcinoma) and normal tissue closely adjacent to tumor.MiRNA-449a simulation was designed and synthesized, was dissolved into two different concentrations as 10 and 20 mg/mL.The expression of miRNA-449a in lung cancer tissues and matched normal tissues were detected by Real time PCR .The expression of luciferase gene was detected by chemiluminescence technique.MiRNA-449a mimics on cell apoptosis was evaluated by MTT assay . Results The mean tissues expression levels of miRNA-449 a in squamous carcinoma group and adenocarcinoma group were 1.48 ±1.63 and 1.52 ±1.54 respectively, and were significantly lower than in control group (2.74 ± 1.55 ) ( P<0.01 ) .The average intensity of fluorescent protein in 10 mg/mL group and 20 mg/mL group were 2 115 ±168 and 1 352 ±159 respectively , and were significantly lower than that in control group ( 4 975 ±115 ) ( P<0.01 ) .Conclusions MiRNA-449 a was down-regulated expression in lung cancer and induced apoptosis .
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AIM: To observe clinical efficacy of Tanreqing Injection (Radix Scutellariae, Bear gall powder, Ram's horn, Flos lonicerae japonicae and Fructus forsythiae) in treatment of patients with acute attack of bronchial asthma. METHODS: One hundred and seven patients with an acute asthma were randomly assigned into two groups: a treatment group (n=52, treated by Tanreqing Injection for 10 d in addition to the routine medications) and a control group (n=55, treated by routine medications). The serum levels of ET and TNF-α were determined by radioimmunoassay before and after treatment, respectively. RESULTS: Before treatment, there were no significant differences in the content of serum ET and TNF-α between the treatment group and the control group. However, after 10 d of treatment, the levels of ET and TNF-α inthe treatment group were significantly lower than those of the control group (P<0.01), and clinical efficacy of the treatment group was superior to the control group. CONCLUSION: Inhibitionof ET and TNF-α secretion and intervention inflammatory response might be one of mechanisms of Tanreqing Injection in treatment for acute asthma.
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Objective To explore the diagnosis rate.pathology types and positive rate of cancer cell in spu-tum of early central pulmonary carcinoma in three obstructive signs on chest X ray screened by fiberbromchoscope.Methods 326 cases of three obstructive signs with high risk of lung cancer were screened for central pulmonarycarcinoma by spiral CT.biopsy by fiberbronchoscope and cytological examination of sputum.Results 32 patients were diagnosed with central pulmonary carcinoma,with morbidity of 9.8%.In these patients,21 were confirmed with obstructive pneumonia(65.6%),7 with obstructive atelectasis(21.9%),4 with obstructive emphysema(12.5%);In terms of pathology type,16 cases were defined as squamous cell carcinoma(50.0%),9 as small cell carcinoma(28.1%).3 were as large cell carcinoma(9.4%).2 were as adenocarcinoma(6.3%),1 as admosquamous carci-noma(3.1%),1 as bronchial gland carcinoma(3.1%);cancer cell could be found in sputum of 5 patients of 32 cases,among them,it was found in 3 of 21 patients with obstructive pneumonia(14.3%),1 in 7 patients with ob-structive atelectasis(14.3%),1 in 4 patients with obstructive emphysema(25.0%).Conclusion The prevelance of early central pulmonary carcinoma in three obstructive signs on chest X-ray is 9.815%,in which squamous carci-noma and small-cell carcinoma are common in pathology type.Screening can increase the detection rate of early pul-monary carcinoma.
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AIM: To observe clinical efficacy of Tanreqing Injection(Radix Scutellariae,Bear gall powder,Ram's horn,Flos lonicerae japonicae and Fructus forsythiae) in treatment of patients with acute attack of bronchial asthma. METHODS: One hundred and seven patients with an acute asthma were randomly assigned into two groups: a treatment group(n=52,treated by Tanreqing Injection for 10 d in addition to the routine medications) and a control group (n=55,treated by routine medications).The serum levels of ET and TNF-? were determined by radioimmunoassay before and after treatment,respectively. RESULTS: Before treatment,there were no significant differences in the content of serum ET and TNF-? between the treatment group and the control group.However,after 10 d of treatment,the levels of ET and TNF-? in the treatment group were significantly lower than those of the control group(P