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<p><b>OBJECTIVE</b>To explore the effects of electroacupuncture (EA) combined with motor imagery therapy on motor function and activity of daily living in patients with hemiplegic cerebral infarction.</p><p><b>METHODS</b>Ninety patients with hemiplegic cerebral infarction were randomly divided into a rehabilitation group, an EA group and a comprehensive group, 30 patients in each one. The patients in the rehabilitation group were treated with regular care, medication and rehabilitation training; based on the rehabilitation group, the patients in the EA group were treated mainly with electroacupuncture at Baihui (GV 20), Dingnieqianxiexian (MS 6), Dingniehouxiexian (MS 7), Jianyu (LI 15), Waiguan (TE 5), Fengshi (GB 31) and Sanyinjiao (SP 6); with the arrival of. EA device was connected for 30 min. The patients in the comprehensive group were treated with EA as the EA group and motor imagery therapy, 20 min per treatment. The treatment was given once a day, five treatments per week, and totally 4-week treatment was performed. The Barthel index and Brunnstrom score before and after treatment were observed in the three groups.</p><p><b>RESULTS</b>Three cases did not finish the trial and finally 87 cases were included into analysis, including 30 cases in the rehabilitation group, 29 cases in the EA group and 28 cases in the comprehensive group. Compared before treatment, the Barthel index and Brunnstrom score were significantly improved after treatment in the three groups (all<0.01); after treatment, the Barthel index in the EA group and comprehensive group was significantly higher than that in the rehabilitation group (both<0.01); the lower extremity score of Brunnstrom score in the comprehensive group was better than those in the EA group and rehabilitation group (both<0.05).</p><p><b>CONCLUSION</b>EA combined with motor imagery therapy and rehabilitation can significantly improve the motor function and activity of daily living in patients with hemiplegic cerebral infarction, which is superior to rehabilitation alone or EA alone.</p>
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Objective:To explore the role of miR-214 in the progression of hepatocellular carcinoma (HCC) and its inhibitory mechanisms in depressing the signaling pathway ofβ-catenin, this study was conducted.Methods:We ectopically expressed miR-214 in HepG2 cells to obtain cell lines Lv-miR-214-HepG2 and their control Lv-control-HepG2. Differences between the two cell lines were compared in cell growth, proliferation, colony forming ability and cell cycles. RT-PCR method was applied for the quantification ofβ-catenin mRNA expression. Western-blot method was applied for the determination of the protein level ofβ-catenin and their downstream targets (ie. Cyclin D1, c-Myc and TCF-1). The effect of miR-214 on cells was further explored through RNA interference and restoring miR-214 expression.Results:In comparison with negative (Lv-control-HepG2) and blank (HepG2) control, a significant inhibition of cell growth and proliferation caused by miR-214 was observed after 48~72h of cell culture experiments (P0.05). By comparing to the RT-PCR and Western-blot results of control, the miR-214 treatment led to a slightly decrease in theβ-catenin mRNA expression (P>0.05), but an extremely inhibition in the protein level ofβ-catenin and its downstream targets Cyclin D1, c-Myc, and TCF-1 (P<0.05).Conclusions:miR-214 functions as a suppressor during the progression of HCC, and its inhibitory role was achieved by down-regulatingβ-catenin signaling pathway.
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Objective To probe into the curative effect and functional mechanism of acupuncture treatment on obesity complicated with hyperlipidemia.Methods The 60 cases of simple obesity complicated with hyperlipidemia were randomized into treatment group and control group with 30 in each.The treatment group was treated with acupuncture only while the control group with routine medicinal therapy.Before and after treatment,the blood-fat index and symptoms and signs of patients were observed.The comparison of two groups after 3 courses of treatment was made to study the functions of acupuncture for obesity.Results In the treatment group,there showed regulated blood-fat levels and the decrease of triglyceride(TG) was significant.In the control group,there also showed regulated blood-fat levels to different degrees,and the difference in levels of total cholesterol(TC),low density lipoprotein(LDL),and apolipoprotein-B(ApoB) before and after treatment was significant.The TCM symptom scores of two groups were statistically different.Conclusion Acupuncture is effective for the simple obesity complicated with hyperlipidemia.